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Journal of Clinical Microbiology, January 1998, p. 15-19, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Use of Ribotyping and Hemolysin Activity To Identify Highly Virulent Streptococcus suis Type 2 Isolatesdagger

Jacque J. Staats,1 Brandon L. Plattner,1 Jerome Nietfeld,1 Steve Dritz,2 and M. M. Chengappa1,*

Department of Diagnostic Medicine/Pathobiology1 and the Food Animal Health and Management Center,2 Kansas State University, Manhattan, Kansas 66506

Received 18 August 1997/Accepted 1 October 1997

Nineteen Streptococcus suis type 2 isolates were evaluated for their virulence in pigs and mice. Of these, seven were determined to be highly virulent in pigs on the basis of clinical sign scores and gross pathology and histopathology results. Clinical sign scores correlated with gross pathology and histopathology scores at P equal to 0.004 and P equal to 0.009, respectively. The virulence of highly virulent isolates in pigs compared somewhat with virulence in mice, but the correlation was not significant. No correlation of virulence was noted among the moderately virulent and avirulent isolates in pigs and mice. Chromosomal DNAs from all S. suis isolates were evaluated by PstI, PvuII, EcoRI, and HaeIII restriction enzyme digestion followed by hybridization with a digoxigenin-11-dUTP-labeled cDNA probe transcribed from 16S and 23S rRNAs from Escherichia coli. The hybridization patterns (ribotypes) varied depending upon the enzyme used, but a significant number of isolates determined to be highly virulent in pigs had unique hybridization patterns compared with those of the moderately virulent and avirulent isolates (P = 0.002). In addition, hemolysin activity showed a high correlation to virulence (P = 0.00008) and ribotype (P = 0.002).

* Corresponding author. Mailing address: Department of Diagnostic Medicine/Pathobiology, 310 VMS, College of Veterinary Medicine, Manhattan, KS 66506-5606. Phone: (913) 532-4605. Fax: (913) 532-4039. E-mail: Chengapa{at}vet.ksu.edu.

dagger Kansas Agricultural Experiment Station contribution no. 97-413-J.

Journal of Clinical Microbiology, January 1998, p. 15-19, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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