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Journal of Clinical Microbiology, January 1998, p. 273-274, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evaluation of Screening and Commercial Methods for Detection of Methicillin Resistance in Coagulase-Negative Staphylococci

Zafar Hussain,1,2,* Luba Stoakes,1 Robert Lannigan,1,2 Susan Longo,1 and Barbara Nancekivell1

Department of Microbiology, London Health Sciences Centre,1 and Department of Microbiology and Immunology, University of Western Ontario,2 London, Ontario, Canada

Received 26 August 1997/Accepted 22 September 1997

The National Committee for Clinical Laboratory Standards recommends 48 h of incubation by the oxacillin salt agar screen (OSAS) method for the detection of methicillin-resistant coagulase-negative staphylococci (CoNS). An earlier identification of methicillin resistance is desirable. The time to detection of the mecA gene by PCR was compared with the times to detection by OSAS, by the oxacillin disk diffusion (ODD) method, and with MicroScan Gram Positive Combo type 6 panels (MicroScan Inc. Sacramento, Calif.) and Vitek GPS-SA cards (bioMérieux Vitek Inc., Hazelwood, Mo.). The combination of the Vitek card and the ODD method detected 92 of 99 methicillin-resistant strains of CoNS at 24 h; however, 6 mecA-positive strains were phenotypically methicillin susceptible. We conclude that most methicillin-resistant CoNS can be detected and the results can be reported after overnight incubation by a combination of methods.

* Corresponding author. Mailing address: Department of Microbiology and Infection Control, London Health Sciences Centre, Box 5375, London, Ontario, Canada N6A 4G5. Phone: (519) 685-8149. Fax: (519) 685-8203. E-mail: Zafar.Hussain{at}lhsc.on.ca.

Journal of Clinical Microbiology, January 1998, p. 273-274, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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