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Journal of Clinical Microbiology, January 1998, p. 311-314, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Absolute Copy Number and Relative Change in Determinations of Human Immunodeficiency Virus Type 1 RNA in Plasma: Effect of an External Standard on Kit Comparisons

Donald Brambilla,1 Sheila Leung,1 Judy Lew,2 John Todd,3 Steven Herman,4 Mike Cronin,5 David E. Shapiro,6 James Bremer,7 Celine Hanson,8 George V. Hillyer,9 George D. McSherry,10 Rhoda S. Sperling,11 Robert W. Coombs,12 and Patricia S. Reichelderfer2,*

New England Research Institutes, Watertown, Massachusetts 021721; National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 208922; Chiron Corporation, Emeryville, California 946083; Roche Molecular Systems, Branchburg, New Jersey 088764; Organon Teknika Corporation, Durham, North Carolina 277125; Harvard School of Public Health, Boston, Massachusetts 021156; Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 606127; Baylor College of Medicine, Houston, Texas 770308; University of Puerto Rico School of Medicine, San Juan, Puerto Rico 00936-50679; UMD-New Jersey Medical School, Newark, New Jersey 0710310; Mount Sinai Medical Center, New York, New York 1002911; and University of Washington, Seattle, Washington 9819512

Received 13 June 1997/Returned for modification 18 August 1997/Accepted 27 October 1997

Use of a common set of human immunodeficiency virus type 1 (HIV-1) RNA standards eliminated differences among absolute HIV-1 RNA copy number estimates made with three commercially available assays. The relative changes in the viral RNA levels determined by the commercial assays were similar and were unaffected by the use of a common set of standards.

* Corresponding author. Mailing address: NIH/NIAID/DAIDS, 6003 Executive Blvd., Rockville, MD 20776. Phone: (301) 496-0636. Fax: (301) 402-3171. E-mail: pr20f{at}nih.gov.

Journal of Clinical Microbiology, January 1998, p. 311-314, Vol. 36, No. 1
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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