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Journal of Clinical Microbiology, October 1998, p. 3013-3019, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Plaque Formation by and Plaque Cloning of Chlamydia trachomatis Biovar Trachoma

Akira Matsumoto,1,* Hiroshi Izutsu,2 Naoyuki Miyashita,3 and Masanobu Ohuchi1

Department of Microbiology1 and Division of Respiratory Diseases, Department of Medicine,3 Kawasaki Medical School, Kurashiki, Okayama 701-0192, and Pharmaceutical Research Laboratory, Hitachi Chemical Co. Ltd., Hitachi, Ibaragi 317,2 Japan

Received 12 March 1998/Returned for modification 5 May 1998/Accepted 30 June 1998

A new technique for the induction of plaque formation by Chlamydia trachomatis biovar trachoma applicable to the titration of infectivity and cloning of biovar trachoma was established. Three novel strains were cloned and confirmed to be free of glycogen inclusions. The lack of glycogen accumulation correlated with the absence of a 7.5-kb plasmid, which is highly conserved in other strains of C. trachomatis. Although the growth efficiency of these plasmid-free strains was slightly lower than that of plasmid-positive strains, possession of the plasmid and glycogen accumulation were not essential for the survival of C. trachomatis.

* Corresponding author. Mailing address: Department of Microbiology, Kawasaki Medical School, Kurashiki, Okayama 701-0192, Japan. Phone: 086-462-1111. Fax: 086-462-1199. E-mail: akimat{at}med.kawasaki-m.ac.jp.

Journal of Clinical Microbiology, October 1998, p. 3013-3019, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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