This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cao, L.
Right arrow Articles by Yuen, K.-Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cao, L.
Right arrow Articles by Yuen, K.-Y.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, October 1998, p. 3028-3031, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Specific Antibodies to an Antigenic Mannoprotein for Diagnosis of Penicillium marneffei Penicilliosis

Liang Cao,1,* Da-Liang Chen,1 Cindy Lee,1 Che-Man Chan,1 King-Man Chan,1 Nongnuch Vanittanakom,2 Dominic N. C. Tsang,3 and Kwok-Yung Yuen1

Department of Microbiology, The University of Hong Kong,1 and Department of Pathology, Queen Elizabeth Hospital,3 Hong Kong, and Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand2

Received 30 March 1998/Returned for modification 27 May 1998/Accepted 14 July 1998

The disseminated and progressive fungal disease Penicillium marneffei penicilliosis is one of the most common infectious diseases in AIDS patients in Southeast Asia. To diagnose systemic penicilliosis, we developed an enzyme-linked immunosorbent assay (ELISA)-based antibody test with Mp1p, a purified recombinant antigenic mannoprotein of P. marneffei. Evaluation of the test with guinea pig sera against P. marneffei and other pathogenic fungi indicated that this assay was specific for P. marneffei. Clinical evaluation revealed that high levels of specific antibody were detected in two immunocompetent penicilliosis patients. Furthermore, approximately 80% (14 of 17) of the documented penicilliosis patients with human immunodeficiency virus tested positive for the specific antibody. No false-positive results were found for serum samples from 90 healthy blood donors, 20 patients with typhoid fever, and 55 patients with tuberculosis, indicating a high specificity of the test. Thus, this ELISA-based test for the detection of anti-Mp1p antibody can be of significant value as a diagnostic for penicilliosis.

* Corresponding author. Mailing address: Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital Compound, Hong Kong. Phone: (852) 2855-4822. Fax: (852) 2855-1241. E-mail: lcao{at}hkucc.Hku.hk.

Journal of Clinical Microbiology, October 1998, p. 3028-3031, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Martin-Davila, P., Fortun, J., Lopez-Velez, R., Norman, F., Montes de Oca, M., Zamarron, P., Gonzalez, M. I., Moreno, A., Pumarola, T., Garrido, G., Candela, A., Moreno, S. (2008). Transmission of Tropical and Geographically Restricted Infections during Solid-Organ Transplantation. Clin. Microbiol. Rev. 21: 60-96 [Abstract] [Full Text]  
  • Woo, P. C. Y., Lau, C. C. Y., Chong, K. T. K., Tse, H., Tsang, D. N. C., Lee, R. A., Tse, C. W. S., Que, T.-l., Chung, L. M. W., Ngan, A. H. Y., Hui, W.-t., Wong, S. S. Y., Lau, S. K. P., Yuen, K.-y. (2007). MP1 Homologue-Based Multilocus Sequence System for Typing the Pathogenic Fungus Penicillium marneffei: a Novel Approach Using Lineage-Specific Genes. J. Clin. Microbiol. 45: 3647-3654 [Abstract] [Full Text]  
  • Lasker, B. A. (2006). Nucleotide Sequence-Based Analysis for Determining the Molecular Epidemiology of Penicillium marneffei.. J. Clin. Microbiol. 44: 3145-3153 [Abstract] [Full Text]  
  • Vanittanakom, N., Cooper, C. R. Jr., Fisher, M. C., Sirisanthana, T. (2006). Penicillium marneffei Infection and Recent Advances in the Epidemiology and Molecular Biology Aspects. Clin. Microbiol. Rev. 19: 95-110 [Abstract] [Full Text]  
  • Chong, K. T. K., Woo, P. C. Y., Lau, S. K. P., Huang, Y., Yuen, K.-y. (2004). AFMP2 Encodes a Novel Immunogenic Protein of the Antigenic Mannoprotein Superfamily in Aspergillus fumigatus. J. Clin. Microbiol. 42: 2287-2291 [Abstract] [Full Text]  
  • Woo, P. C. Y., Lau, S. K. P., Wong, B. H. L., Tsoi, H.-w., Fung, A. M. Y., Chan, K.-h., Tam, V. K. P., Peiris, J. S. M., Yuen, K.-y. (2004). Detection of Specific Antibodies to Severe Acute Respiratory Syndrome (SARS) Coronavirus Nucleocapsid Protein for Serodiagnosis of SARS Coronavirus Pneumonia. J. Clin. Microbiol. 42: 2306-2309 [Abstract] [Full Text]  
  • Woo, P. C. Y., Chong, K. T. K., Leung, A. S. P., Wong, S. S. Y., Lau, S. K. P., Yuen, K.-Y. (2003). AFLMP1 Encodes an Antigenic Cell Wall Protein in Aspergillus flavus. J. Clin. Microbiol. 41: 845-850 [Abstract] [Full Text]  
  • Woo, P. C. Y., Chan, C.-M., Leung, A. S. P., Lau, S. K. P., Che, X.-Y., Wong, S. S. Y., Cao, L., Yuen, K.-Y. (2002). Detection of Cell Wall Galactomannoprotein Afmp1p in Culture Supernatants of Aspergillus fumigatus and in Sera of Aspergillosis Patients. J. Clin. Microbiol. 40: 4382-4387 [Abstract] [Full Text]  
  • Desakorn, V., Simpson, A. J. H., Wuthiekanun, V., Sahassananda, D., Rajanuwong, A., Pitisuttithum, P., Howe, P. A., Smith, M. D., White, N. J. (2002). Development and Evaluation of Rapid Urinary Antigen Detection Tests for Diagnosis of Penicilliosis Marneffei. J. Clin. Microbiol. 40: 3179-3183 [Abstract] [Full Text]  
  • Yeo, S. F., Wong, B. (2002). Current Status of Nonculture Methods for Diagnosis of Invasive Fungal Infections. Clin. Microbiol. Rev. 15: 465-484 [Abstract] [Full Text]  
  • Chan, C.-m., Woo, P. C. Y., Leung, A. S. P., Lau, S. K. P., Che, X.-y., Cao, L., Yuen, K.-y. (2002). Detection of Antibodies Specific to an Antigenic Cell Wall Galactomannoprotein for Serodiagnosis of Aspergillus fumigatus Aspergillosis. J. Clin. Microbiol. 40: 2041-2045 [Abstract] [Full Text]  
  • Vanittanakom, N., Vanittanakom, P., Hay, R. J. (2002). Rapid Identification of Penicillium marneffei by PCR-Based Detection of Specific Sequences on the rRNA Gene. J. Clin. Microbiol. 40: 1739-1742 [Abstract] [Full Text]  
  • Wong, S. S. Y., Wong, K. H., Hui, W. T., Lee, S. S., Lo, J. Y. C., Cao, L., Yuen, K. Y. (2001). Differences in Clinical and Laboratory Diagnostic Characteristics of Penicilliosis Marneffei in Human Immunodeficiency Virus (HIV)- and Non-HIV-Infected Patients. J. Clin. Microbiol. 39: 4535-4540 [Abstract] [Full Text]  
  • Woo, P. C. Y., Leung, A. S. P., Lau, S. K. P., Chong, K. T. K., Yuen ;, K.-Y., Weig, M., Gro{beta}, U., Domhof, S., Brunner, E. (2001). Use of Recombinant Mitogillin for Serodiagnosis of Aspergillus fumigatus-Associated Diseases. J. Clin. Microbiol. 39: 4598-4600 [Full Text]  
  • Yuen, K.-Y., Chan, C.-M., Chan, K.-M., Woo, P. C. Y., Che, X.-Y., Leung, A. S. P., Cao, L. (2001). Characterization of AFMP1: a Novel Target for Serodiagnosis of Aspergillosis. J. Clin. Microbiol. 39: 3830-3837 [Abstract] [Full Text]  
  • Cao, L., Chan, K.-M., Chen, D., Vanittanakom, N., Lee, C., Chan, C.-M., Sirisanthana, T., Tsang, D. N. C., Yuen, K.-Y. (1999). Detection of Cell Wall Mannoprotein Mp1p in Culture Supernatants of Penicillium marneffei and in Sera of Penicilliosis Patients. J. Clin. Microbiol. 37: 981-986 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»