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Journal of Clinical Microbiology, October 1998, p. 3105-3110, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Extended-Spectrum beta -Lactamase-Producing Klebsiella pneumoniae Strains Causing Nosocomial Outbreaks of Infection in the United Kingdom

Kevin Shannon,1,* Paul Stapleton,1 Xiaoqin Xiang,1,dagger Alan Johnson,2 Hamish Beattie,2 Fatima El Bakri,2,Dagger Barry Cookson,2 and Gary French1

Department of Microbiology, United Medical and Dental Schools, St. Thomas's Campus, London SE1 7EH,1 and Laboratory of Hospital Infection, PHLS Central Public Health Laboratory, London NW9 5HT,2 United Kingdom

Received 21 January 1998/Returned for modification 12 May 1998/Accepted 9 July 1998

Representative isolates from 10 distinct extended-spectrum beta -lactamase-producing strains of Klebsiella pneumoniae that caused hospital outbreaks in the United Kingdom from 1991 to 1994 were examined for relationships between their enzymes and plasmids. The beta -lactamases were identified by a combination of isoelectric focusing and gene sequencing. SHV-2 beta -lactamase was produced by isolates from four outbreaks, SHV-5 was involved in three, and SHV-4, TEM-15, and TEM-26 were involved in one outbreak each. All of the extended-spectrum beta -lactamases were encoded by self-transmissible plasmids, with sizes ranging from about 70 to 160 kb. No similarities between the restriction digest patterns of the extended-spectrum beta -lactamase-encoding plasmids were detected, except to some extent between those that produced TEM-15 and TEM-26. Thus, outbreaks of hospital infection with these organisms in the United Kingdom from 1991 to 1994 involved distinct organisms and resistance plasmids and appeared to be unrelated.

* Corresponding author. Mailing address: Department of Microbiology, United Medical and Dental Schools, St. Thomas's Campus, London SE1 7EH, United Kingdom. Phone: 44-171 922 8383. Fax: 44-171 928 0730. E-mail: k.shannon{at}umds.ac.uk.

dagger Present address: Department of Infectious Diseases, Chongqing University of Medical Sciences, Chongqing 630042, People's Republic of China.

Dagger Present address: Southampton Public Health Laboratory, Southampton SO16 6YD, United Kingdom.

Journal of Clinical Microbiology, October 1998, p. 3105-3110, Vol. 36, No. 10
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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