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Journal of Clinical Microbiology, November 1998, p. 3170-3172, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Diagnostic Potential of Toscana Virus N Protein Expressed in Escherichia coli

Marcello Valassina,1 Dario Soldateschi,2 Grazia Maria Dal Maso,2 Laura Santini,1 Silvia Bianchi,1 Pier Egisto Valensin,1 and Maria Grazia Cusi1,*

Department of Molecular Biology, Section of Microbiology, University of Siena, 53100 Siena,1 and DIESSE Srl Via delle Rose 10, 53035 Monteriggioni (SI),2 Italy

Received 22 May 1998/Returned for modification 13 July 1998/Accepted 17 August 1998

The nucleocapsid (N) protein of the Toscana (TOS) virus was expressed in Escherichia coli by using a pET15b vector. The recombinant protein was purified by affinity chromatography and was characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, immunoblotting, and enzyme immunoassay (EIA). The recombinant antigen was reactive with positive human sera, and the reactivity correlated very well (r = 0.9) with that of a whole-virus antigen when tested by EIA with 30 TOS virus-positive and 30 TOS virus-negative serum samples. The results demonstrate that the recombinant N protein can be easily produced in a procaryotic system and used for diagnostic assays for TOS virus immunity.

* Corresponding author. Mailing address: Department of Molecular Biology, Section of Microbiology, University of Siena, 53100 Siena, Italy. Phone: 0039 577 263850. Fax: 0039 577 263870. E-mail: Cusi{at}unisi.it.

Journal of Clinical Microbiology, November 1998, p. 3170-3172, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Valassina, M., Valentini, M., Pugliese, A., Valensin, P. E., Cusi, M. G. (2003). Serological Survey of Toscana Virus Infections in a High-Risk Population in Italy. CVI 10: 483-484 [Abstract] [Full Text]  
  • Saeed, M. F., Nunes, M., Vasconcelos, P. F., Travassos Da Rosa, A. P. A., Watts, D. M., Russell, K., Shope, R. E., Tesh, R. B., Barrett, A. D. T. (2001). Diagnosis of Oropouche Virus Infection Using a Recombinant Nucleocapsid Protein-Based Enzyme Immunoassay. J. Clin. Microbiol. 39: 2445-2452 [Abstract] [Full Text]  
  • Soldateschi, D., Dal Maso, G. M., Valassina, M., Santini, L., Bianchi, S., Cusi, M. G. (1999). Laboratory Diagnosis of Toscana Virus Infection by Enzyme Immunoassay with Recombinant Viral Nucleoprotein. J. Clin. Microbiol. 37: 649-652 [Abstract] [Full Text]  


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