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Journal of Clinical Microbiology, November 1998, p. 3178-3181, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Enzyme-Linked Immunosorbent Assay Based on Recombinant Human Group C Rotavirus Inner Capsid Protein (VP6) To Detect Human Group C Rotaviruses in Fecal Samples

V. L. A. James,1,* P. R. Lambden,2 E. O. Caul,3 and , and I. N. Clarke2

Public Health Laboratory1 and Department of Molecular Microbiology, University of Southampton,2 Southampton General Hospital, Southampton SO16 6YD, and Public Health Laboratory, Kingsdown, Bristol BS2 8HW,3 United Kingdom

Received 28 April 1998/Returned for modification 6 July 1998/Accepted 18 August 1998

A recent study showed that 43% of a population in the United Kingdom were seropositive for group C rotavirus. The higher than expected incidence may be due to limited diagnosis of acute human group C rotavirus infections because no routine test is available. Human group C rotavirus infections are routinely diagnosed by electron microscopy (EM) and a negative group A rotavirus enzyme-linked immunosorbent assay (ELISA) result. An antigen-detection ELISA was developed with hyperimmune antibodies raised to human group C rotavirus recombinant VP6 (Bristol strain) expressed in insect cells. The assay was used to screen fecal samples to determine the prevalence of group C rotavirus infection. Samples positive by ELISA were confirmed by EM, polyacrylamide gel electrophoresis of double-stranded RNA, or detection of the VP6 gene by reverse transcription-PCR. Retrospective analysis indicated a 1 to 2% detection rate of positivity among samples from patients with acute diarrhea.

* Corresponding author. Mailing address: Public Health Laboratory, Southampton General Hospital, Southampton SO16 6YD, United Kingdom. Phone: 44 1703 798760. Fax: 44 1703 774316. E-mail: vlaj{at}soton.ac.uk.

Journal of Clinical Microbiology, November 1998, p. 3178-3181, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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