This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow An author's correction has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ramachandran, M.
Right arrow Articles by the National Rotavirus Strain Surveillance System Collaborating Laboratories
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ramachandran, M.
Right arrow Articles by the National Rotavirus Strain Surveillance System Collaborating Laboratories,

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 1998, p. 3223-3229, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection and Characterization of Novel Rotavirus Strains in the United States

M. Ramachandran,1 J. R. Gentsch,1,* U. D. Parashar,1,2 S. Jin,1 P. A. Woods,1 J. L. Holmes,1 C. D. Kirkwood,1 R. F. Bishop,3 H. B. Greenberg,4 S. Urasawa,5 G. Gerna,6 B. S. Coulson,7 K. Taniguchi,8 J. S. Bresee,1 R. I. Glass,1 and the National Rotavirus Strain Surveillance System Collaborating Laboratoriesdagger

Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases,1 and Epidemic Intelligence Service, Epidemiology Program Office,2 Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Gastroenterology, Royal Children's Hospital, Melbourne,3 and Department of Microbiology and Immunology, The University of Melbourne, Parkville, Victoria,7 Australia; Division of Gastroenterology, Stanford University School of Medicine, Stanford, and The Palo Alto Veterans Administration Hospital, Palo Alto, California4; Virus Laboratory, Department of Hygiene, Sapporo Medical University, Sapporo,5 and Department of Virology and Parasitology, Fujita Health University, School of Medicine, Aichi,8 Japan; and Viral Diagnostic Service, IRCCS Policlinico San Mateo, Pavia, Italy6

Received 23 April 1998/Returned for modification 8 July 1998/Accepted 11 August 1998

We recently established a rotavirus strain surveillance system in the United States to monitor the prevalent G serotypes before and after the anticipated implementation of a vaccination program against rotavirus and to identify the emergence of uncommon strains. In this study, we examined 348 rotavirus strains obtained in 1996 to 1997 from children with diarrhea in 10 U.S. cities. Strains were characterized for P and G types, subgroups, and electropherotypes by using a combination of monoclonal antibody immunoassay, reverse transcription-PCR, and hybridization. The four strains most commonly found worldwide comprised 83% of the isolates (P[8]G1, 66.4%; P[4]G2, 8.3%; P[8]G3, 6.9%; P[8]G4, 1.4%), but 9.2% were unusual strains (P[6]G9, 5.5%; P[8]G9, 1.7%; P[6]G1, 1.4%; and P[4]G1 and P[8]G2, 0.3% each). Strains not typeable for P or G type accounted for 5.5% of the total, while 2.3% of the strains had more than one G type (mixed infections). All P[6]G9 strains tested had short electropherotypes and subgroup I specificity and were detected in 4 of 10 cities, while P[8]G9 strains had long electropherotypes and subgroup II VP6 antigens. Both sequence analysis of the VP7 open reading frame (about 94 to 95% amino acid identity with the VP7 gene of G9 prototype strain WI61) and binding to a G9-specific monoclonal antibody strongly suggest that U.S. G9 strains belong to serotype G9. The high detection rates of unusual rotaviruses with G9 (7.2%) or P[6] (6.9%) specificity in multiple U.S. cities suggest the emergence of new strains or inadequate diagnosis in the past. The epidemiologic importance of these strains remains to be determined.

* Corresponding author. Mailing address: Viral Gastroenteritis Section MS G04, Centers for Disease Control and Prevention, 1600 Clifton Rd., N.E., Atlanta, GA 30333. Phone: (404) 639-3577. Fax: (404) 639-3645. E-mail: jrg4{at}cdc.gov.

dagger The National Rotavirus Strain Surveillance System participants are Rebecca Nelson, Arkansas Children's Hospital, Little Rock, Ark.; Michelle Hartin, Children's Hospital, San Diego, Calif.; Christine C. Robinson, Children's Hospital of Denver, Denver, Colo.; Yolanda Arcilla, Medical Center of Delaware, Newark, Del.; Theresa J. Mason, Clarian Health Partners, Indianapolis, Ind.; Marilyn Hamilton, Children's Mercy Hospital, Kansas City, Mo.; Paul A. Yam, Children's Hospital, Omaha, Nebr.; DeLores Aiazzi, Washoe Medical Center, Reno, Nev.; H. Fred Clark, Children's Hospital of Philadelphia, Philadelphia, Pa.; and Tammi Johnson, Driscoll Children's Hospital, Corpus Christi, Tex.

Journal of Clinical Microbiology, November 1998, p. 3223-3229, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kiulia, N. M., Nyaundi, J. K., Peenze, I., Nyachieo, A., Musoke, R. N., Steele, A. D., Mwenda, J. M. (2009). Rotavirus Infections among HIV-Infected Children in Nairobi, Kenya. J Trop Pediatr 55: 318-323 [Abstract] [Full Text]  
  • Bourdett-Stanziola, L., Jimenez, C., Ortega-Barria, E. (2008). Diversity of Human Rotavirus G and P Genotypes in Panama, Costa Rica, and the Dominican Republic. Am J Trop Med Hyg 79: 921-924 [Abstract] [Full Text]  
  • Hoshino, Y., Jones, R. W., Ross, J., Honma, S., Santos, N., Gentsch, J. R., Kapikian, A. Z. (2004). Rotavirus Serotype G9 Strains Belonging to VP7 Gene Phylogenetic Sequence Lineage 1 May Be More Suitable for Serotype G9 Vaccine Candidates than Those Belonging to Lineage 2 or 3. J. Virol. 78: 7795-7802 [Abstract] [Full Text]  
  • Iturriza Gomara, M., Kang, G., Mammen, A., Jana, A. K., Abraham, M., Desselberger, U., Brown, D., Gray, J. (2004). Characterization of G10P[11] Rotaviruses Causing Acute Gastroenteritis in Neonates and Infants in Vellore, India. J. Clin. Microbiol. 42: 2541-2547 [Abstract] [Full Text]  
  • Clark, H. F., Lawley, D. A., Schaffer, A., Patacsil, J. M., Marcello, A. E., Glass, R. I., Jain, V., Gentsch, J. (2004). Assessment of the Epidemic Potential of a New Strain of Rotavirus Associated with the Novel G9 Serotype Which Caused an Outbreak in the United States for the First Time in the 1995-1996 Season. J. Clin. Microbiol. 42: 1434-1438 [Abstract] [Full Text]  
  • Sanchez-Fauquier, A., Wilhelmi, I., Colomina, J., Cubero, E., Roman, E. (2004). Diversity of Group A Human Rotavirus Types Circulating over a 4-Year Period in Madrid, Spain. J. Clin. Microbiol. 42: 1609-1613 [Abstract] [Full Text]  
  • Page, N. A., Steele, A. D. (2004). Antigenic and Genetic Characterization of Serotype G2 Human Rotavirus Strains from the African Continent. J. Clin. Microbiol. 42: 595-600 [Abstract] [Full Text]  
  • Wakuda, M., Nagashima, S., Kobayashi, N., Pongsuwanna, Y., Taniguchi, K. (2003). Serologic and Genomic Characterization of a G12 Human Rotavirus in Thailand. J. Clin. Microbiol. 41: 5764-5769 [Abstract] [Full Text]  
  • Graham, K. L., Halasz, P., Tan, Y., Hewish, M. J., Takada, Y., Mackow, E. R., Robinson, M. K., Coulson, B. S. (2003). Integrin-Using Rotaviruses Bind {alpha}2{beta}1 Integrin {alpha}2 I Domain via VP4 DGE Sequence and Recognize {alpha}X{beta}2 and {alpha}V{beta}3 by Using VP7 during Cell Entry. J. Virol. 77: 9969-9978 [Abstract] [Full Text]  
  • Kirkwood, C., Bogdanovic-Sakran, N., Palombo, E., Masendycz, P., Bugg, H., Barnes, G., Bishop, R. (2003). Genetic and Antigenic Characterization of Rotavirus Serotype G9 Strains Isolated in Australia between 1997 and 2001. J. Clin. Microbiol. 41: 3649-3654 [Abstract] [Full Text]  
  • Martella, V., Terio, V., Del Gaudio, G., Gentile, M., Fiorente, P., Barbuti, S., Buonavoglia, C. (2003). Detection of the Emerging Rotavirus G9 Serotype at High Frequency in Italy. J. Clin. Microbiol. 41: 3960-3963 [Abstract] [Full Text]  
  • Laird, A. R., Gentsch, J. R., Nakagomi, T., Nakagomi, O., Glass, R. I. (2003). Characterization of Serotype G9 Rotavirus Strains Isolated in the United States and India from 1993 to 2001. J. Clin. Microbiol. 41: 3100-3111 [Abstract] [Full Text]  
  • Mota-Hernandez, F., Jose Calva, J., Gutierrez-Camacho, C., Villa-Contreras, S., Arias, C. F., Padilla-Noriega, L., Guiscafre-Gallardo, H., Guerrero, M. d. L., Lopez, S., Munoz, O., Contreras, J. F., Cedillo, R., Herrera, I., Puerto, F. I. (2003). Rotavirus Diarrhea Severity Is Related to the VP4 Type in Mexican Children. J. Clin. Microbiol. 41: 3158-3162 [Abstract] [Full Text]  
  • Santos, N., Soares, C. C., Volotao, E. M., Albuquerque, M. C. M., Hoshino, Y. (2003). Surveillance of Rotavirus Strains in Rio de Janeiro, Brazil, from 1997 to 1999. J. Clin. Microbiol. 41: 3399-3402 [Abstract] [Full Text]  
  • Abdel-Haq, N. M., Thomas, R. A., Asmar, B. I., Zacharova, V., Lyman, W. D. (2003). Increased Prevalence of G1P[4] Genotype among Children with Rotavirus-Associated Gastroenteritis in Metropolitan Detroit. J. Clin. Microbiol. 41: 2680-2682 [Abstract] [Full Text]  
  • Iturriza Gomara, M., Wong, C., Blome, S., Desselberger, U., Gray, J. (2002). Molecular Characterization of VP6 Genes of Human Rotavirus Isolates: Correlation of Genogroups with Subgroups and Evidence of Independent Segregation. J. Virol. 76: 6596-6601 [Abstract] [Full Text]  
  • Pongsuwanna, Y., Guntapong, R., Chiwakul, M., Tacharoenmuang, R., Onvimala, N., Wakuda, M., Kobayashi, N., Taniguchi, K. (2002). Detection of a Human Rotavirus with G12 and P[9] Specificity in Thailand. J. Clin. Microbiol. 40: 1390-1394 [Abstract] [Full Text]  
  • O'Halloran, F., Lynch, M., Cryan, B., Fanning, S. (2002). Application of Restriction Fragment Length Polymorphism Analysis of VP7-Encoding Genes: Fine Comparison of Irish and Global Rotavirus Isolates. J. Clin. Microbiol. 40: 524-531 [Abstract] [Full Text]  
  • Das, S., Sen, A., Uma, G., Varghese, V., Chaudhuri, S., Bhattacharya, S. K., Krishnan, T., Dutta, P., Dutta, D., Bhattacharya, M. K., Mitra, U., Kobayashi, N., Naik, T. N. (2002). Genomic Diversity of Group A Rotavirus Strains Infecting Humans in Eastern India. J. Clin. Microbiol. 40: 146-149 [Abstract] [Full Text]  
  • Adah, M. I., Wade, A., Taniguchi, K. (2001). Molecular Epidemiology of Rotaviruses in Nigeria: Detection of Unusual Strains with G2P[6] and G8P[1] Specificities. J. Clin. Microbiol. 39: 3969-3975 [Abstract] [Full Text]  
  • Bok, K., Palacios, G., Sijvarger, K., Matson, D., Gomez, J. (2001). Emergence of G9 P[6] Human Rotaviruses in Argentina: Phylogenetic Relationships among G9 Strains. J. Clin. Microbiol. 39: 4020-4025 [Abstract] [Full Text]  
  • Jain, V., Das, B. K., Bhan, M. K., Glass, R. I., Gentsch, J. R., Indian Strain Surveillance Collaborating Laborator, (2001). Great Diversity of Group A Rotavirus Strains and High Prevalence of Mixed Rotavirus Infections in India. J. Clin. Microbiol. 39: 3524-3529 [Abstract] [Full Text]  
  • Araújo, I. T., Ferreira, M. S. R., Fialho, A. M., Assis, R. M., Cruz, C. M., Rocha, M., Leite, J. P. G. (2001). Rotavirus Genotypes P[4]G9, P[6]G9, and P[8]G9 in Hospitalized Children with Acute Gastroenteritis in Rio de Janeiro, Brazil. J. Clin. Microbiol. 39: 1999-2001 [Abstract] [Full Text]  
  • Cunliffe, N. A., Gondwe, J. S., Graham, S. M., Thindwa, B. D. M., Dove, W., Broadhead, R. L., Molyneux, M. E., Hart, C. A. (2001). Rotavirus Strain Diversity in Blantyre, Malawi, from 1997 to 1999. J. Clin. Microbiol. 39: 836-843 [Abstract] [Full Text]  
  • Bishop, R. F., Masendycz, P. J., Bugg, H. C., Carlin, J. B., Barnes, G. L. (2001). Epidemiological Patterns of Rotaviruses Causing Severe Gastroenteritis in Young Children throughout Australia from 1993 to 1996. J. Clin. Microbiol. 39: 1085-1091 [Abstract] [Full Text]  
  • Santos, N., Volotão, E. M., Soares, C. C., Albuquerque, M. C. M., da Silva, F. M., de Carvalho, T. R. B., Pereira, C. F. A., Chizhikov, V., Hoshino, Y. (2001). Rotavirus Strains Bearing Genotype G9 or P[9] Recovered from Brazilian Children with Diarrhea from 1997 to 1999. J. Clin. Microbiol. 39: 1157-1160 [Abstract] [Full Text]  
  • Iturriza-Gómara, M., Green, J., Brown, D. W. G., Ramsay, M., Desselberger, U., Gray, J. J. (2000). Molecular Epidemiology of Human Group A Rotavirus Infections in the United Kingdom between 1995 and 1998. J. Clin. Microbiol. 38: 4394-4401 [Abstract] [Full Text]  
  • O'Halloran, F., Lynch, M., Cryan, B., O'Shea, H., Fanning, S. (2000). Molecular Characterization of Rotavirus in Ireland: Detection of Novel Strains Circulating in the Population. J. Clin. Microbiol. 38: 3370-3374 [Abstract] [Full Text]  
  • Griffin, D. D., Kirkwood, C. D., Parashar, U. D., Woods, P. A., Bresee, J. S., Glass, R. I., Gentsch, J. R., the National Rotavirus Strain Surveillance System, (2000). Surveillance of Rotavirus Strains in the United States: Identification of Unusual Strains. J. Clin. Microbiol. 38: 2784-2787 [Abstract] [Full Text]  
  • Bon, F., Fromantin, C., Aho, S., Pothier, P., Kohli, E., The Azay Group, (2000). G and P Genotyping of Rotavirus Strains Circulating in France over a Three-Year Period: Detection of G9 and P[6] Strains at Low Frequencies. J. Clin. Microbiol. 38: 1681-1683 [Abstract] [Full Text]  
  • Palombo, E. A., Masendycz, P. J., Bugg, H. C., Bogdanovic-Sakran, N., Barnes, G. L., Bishop, R. F. (2000). Emergence of Serotype G9 Human Rotaviruses in Australia. J. Clin. Microbiol. 38: 1305-1306 [Full Text]  
  • Argüelles, M. H., Villegas, G. A., Castello, A., Abrami, A., Ghiringhelli, P. D., Semorile, L., Glikmann, G. (2000). VP7 and VP4 Genotyping of Human Group A Rotavirus in Buenos Aires, Argentina. J. Clin. Microbiol. 38: 252-259 [Abstract] [Full Text]  
  • Coulson, B. S., Gentsch, J. R., Das, B. K., Bhan, M. K., Glass, R. I. (1999). Comparison of Enzyme Immunoassay and Reverse Transcriptase PCR for Identification of Serotype G9 Rotaviruses. J. Clin. Microbiol. 37: 3187-3193 [Abstract] [Full Text]  
  • Santos, N., Lima, R. C. C., Nozawa, C. M., Linhares, R. E., Gouvea, V. (1999). Detection of Porcine Rotavirus Type G9 and of a Mixture of Types G1 and G5 Associated with Wa-Like VP4 Specificity: Evidence for Natural Human-Porcine Genetic Reassortment. J. Clin. Microbiol. 37: 2734-2736 [Abstract] [Full Text]  
  • RAMSAY, M. (1999). Rotavirus: a new vaccine for the UK?. Arch. Dis. Child. 81: 101-102 [Full Text]  
  • Gault, E., Chikhi-Brachet, R., Delon, S., Schnepf, N., Albiges, L., Grimprel, E., Girardet, J.-P., Begue, P., Garbarg-Chenon, A. (1999). Distribution of Human Rotavirus G Types Circulating in Paris, France, during the 1997-1998 Epidemic: High Prevalence of Type G4. J. Clin. Microbiol. 37: 2373-2375 [Abstract] [Full Text]  
  • Gouvea, V., Santos;, N., Gentsch, J. R., Glass, R. I. (1999). Detection and Characterization of Novel Rotavirus Strains in the United States. J. Clin. Microbiol. 37: 2385-2386 [Full Text]  
  • O'Mahony, J., Foley, B., Morgan, S., Morgan, J. G., Hill, C. (1999). VP4 and VP7 Genotyping of Rotavirus Samples Recovered from Infected Children in Ireland over a 3-Year Period. J. Clin. Microbiol. 37: 1699-1703 [Abstract] [Full Text]  
  • Unicomb, L. E., Podder, G., Gentsch, J. R., Woods, P. A., Hasan, K. Z., Faruque, A. S. G., Albert, M. J., Glass, R. I. (1999). Evidence of High-Frequency Genomic Reassortment of Group A Rotavirus Strains in Bangladesh: Emergence of Type G9 in 1995. J. Clin. Microbiol. 37: 1885-1891 [Abstract] [Full Text]  
  • Crawford, S. E., Estes, M. K., Ciarlet, M., Barone, C., O'Neal, C. M., Cohen, J., Conner, M. E. (1999). Heterotypic Protection and Induction of a Broad Heterotypic Neutralization Response by Rotavirus-Like Particles. J. Virol. 73: 4813-4822 [Abstract] [Full Text]  
  • Nakagomi, T., Horie, Y., Koshimura, Y., Greenberg, H. B., Nakagomi, O. (1999). Isolation of a Human Rotavirus Strain with a Super-Short RNA Pattern and a New P2 Subtype. J. Clin. Microbiol. 37: 1213-1216 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»