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Journal of Clinical Microbiology, November 1998, p. 3255-3259, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Multilocus Genotypic Analysis of Cryptosporidium parvum Isolates from Different Hosts and Geographical Origins

Furio Spano,1 Lorenza Putignani,1 Andrea Crisanti,1,2 Pina Sallicandro,3 Una M. Morgan,4 Sylvie M. Le Blancq,5,6 Laurie Tchack,7 Saul Tzipori,7 and Giovanni Widmer7,*

Istituto di Parassitologia, Università di Roma "La Sapienza," 00185 Rome,1 and Laboratorio di Biologia Cellulare, Istituto Superiore di Sanità, 00161 Rome,3 Italy; Department of Biology, Imperial College of Science, Technology and Medicine, London SW7 2BB, United Kingdom2; World Health Organization Collaborating Center for the Molecular Epidemiology of Infectious Diseases, Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia, 6150 Australia4; Division of Environmental Health Sciences, Columbia University School of Public Health, New York, New York 100325; Center for Environmental Research and Conservation, Columbia University, New York, New York 100276; and Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 015367

Received 8 April 1998/Accepted 20 July 1998

The genetic analysis of oocysts recovered from the stools of humans and animals infected with Cryptosporidium parvum has consistently shown the existence of two distinct genotypes. One of the genotypes is found exclusively in some human infections, whereas the other genotype is found in human as well as in animal infections. On the basis of these observations and the results of published epidemiological studies with single polymorphic markers, the existence of two separate transmission cycles has been postulated, one exclusively anthroponotic and the other involving both animals and humans. To test this hypothesis, C. parvum isolates of different geographic and host origins were analyzed by using unlinked genetic polymorphisms. A total of 28 isolates originating from Europe, North and South America, and Australia were examined. Isolates clustered into two groups, one comprising both human and animal isolates and the other comprising isolates only of human origin. The absence of recombinant genotypes is consistent with two reproductively isolated populations within the species C. parvum.

* Corresponding author. Mailing address: Division of Infectious Diseases, Tufts University School of Veterinary Medicine, 200 Westboro Rd., North Grafton, MA 01536. Phone: (508) 839 7944. Fax: (508) 839 7977. E-mail: gwidmer{at}infonet.tufts.edu.

Journal of Clinical Microbiology, November 1998, p. 3255-3259, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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