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Journal of Clinical Microbiology, November 1998, p. 3359-3361, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Development and Evaluation of an Epstein-Barr Virus (EBV) Immunoglobulin M Enzyme-Linked Immunosorbent Assay Based on the 18-Kilodalton Matrix Protein for Diagnosis of Primary EBV Infection

K. H. Chan,1 R. X. Luo,2 H. L. Chen,3 M. H. Ng,2 W. H. Seto,1 and J. S. M. Peiris2,*

Department of Microbiology, Queen Mary Hospital,1 and The University of Hong Kong,2 Hong Kong, and Department of Pharmacology and Molecular Science, School of Medicine, John Hopkins University, Baltimore, Maryland3

Received 14 May 1998/Returned for modification 7 July 1998/Accepted 19 August 1998

A new immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) based on the recombinant Epstein-Barr virus (EBV) matrix protein was developed. Compared to indirect immunofluorescence for the detection of IgM antibody to the EBV capsid antigen on clinical specimens, the sensitivity and specificity of the new IgM ELISA were 96 and 96%, respectively.

* Corresponding author. Mailing address: Department of Microbiology, University Pathology Building, Queen Mary Hospital Compound, Pokfulam, Hong Kong. Phone: (852) 2855 4888. Fax: (852) 2855 1241. E-mail: microgen{at}hkucc.hku.hk.

Journal of Clinical Microbiology, November 1998, p. 3359-3361, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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