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Journal of Clinical Microbiology, November 1998, p. 3372-3374, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evaluation of the Nuclisens HIV-1 QT Assay for Quantitation of Human Immunodeficiency Virus Type 1 RNA Levels in Plasma

Michel Segondy,1,* Thoai-Duong Ly,2 Martine Lapeyre,2 and Brigitte Montes1

Laboratoire de Virologie, Centre Hospitalier Universitaire de Montpellier, Montpellier,1 and Institut Fournier, Paris,2 France

Received 20 March 1998/Accepted 10 August 1998

Nuclisens HIV-1 QT is a new version of the NASBA HIV-1 QT assay for quantitation of human immunodeficiency virus type 1 (HIV-1) RNA in plasma. The specificity of this assay was 100% in one laboratory and 99%---with nonrepeatability of the initial false positive---in another. The test was linear between 2.0 and 6.0 log RNA copies per ml. According to the input HIV-1 RNA concentration, accuracy varied from -0.11 to +0.10 log RNA copy per ml and precision varied from 0.66 to 0.14 log RNA copy per ml. Reproducibility decreased when the HIV-1 RNA level was near the lower limit of quantitation of the test. HIV-1 RNA could be quantitated by Nuclisens HIV-1 QT in 36% (laboratory 1) and 24% (laboratory 2) of clinical samples with HIV-1 RNA levels lower than the lower limit of quantitation by NASBA HIV-1 QT. Nuclisens HIV-1 QT was not suitable for measurement of RNA from clade G and group O HIV-1 strains.

* Corresponding author. Mailing address: Laboratoire de Virologie, Hôpital Saint-Eloi, Centre Hospitalier Universitaire, 34295 Montpellier Cedex 5, France. Phone: (33) 467-337127. Fax: (33) 467-337623. E-mail: msegondy{at}worldnet.fr.

Journal of Clinical Microbiology, November 1998, p. 3372-3374, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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