This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rappelli, P.
Right arrow Articles by Aceti, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rappelli, P.
Right arrow Articles by Aceti, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 1998, p. 3438-3440, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Development of a Nested PCR for Detection of Cryptococcus neoformans in Cerebrospinal Fluid

Paola Rappelli,1,* Riccardo Are,2 Giuseppe Casu,1 Pier Luigi Fiori,1 Piero Cappuccinelli,1 and Antonio Aceti2

Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology,1 and Institute of Infectious Diseases,2 University of Sassari, Sassari, Italy

Received 23 April 1998/Returned for modification 7 May 1998/Accepted 21 July 1998

We report the development of a nested-PCR-based assay for the detection of Cryptococcus neoformans in cerebrospinal fluid. The specificity and sensitivity of the test were assessed. The technique was then applied to 40 cerebrospinal fluid samples. We obtained positive reactions for all 21 clinical samples from patients who had been previously diagnosed as having cryptococcal meningitis by conventional techniques and negative reactions for all 19 negative controls. Nested PCR is here compared with other diagnostic methods currently used in patients' follow-up exams during anticryptococcal therapy.

* Corresponding author. Mailing address: Department of Biomedical Sciences, Division of Experimental and Clinical Microbiology, University of Sassari, Viale S. Pietro 43/B, 07100 Sassari, Italy. Phone: 39 79 228299. Fax: 39 79 212345. E-mail: micropat{at}ssmain.uniss.it.

Journal of Clinical Microbiology, November 1998, p. 3438-3440, Vol. 36, No. 11
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Saha, D. C., Xess, I., Biswas, A., Bhowmik, D. M., Padma, M. V. (2009). Detection of Cryptococcus by conventional, serological and molecular methods. J Med Microbiol 58: 1098-1105 [Abstract] [Full Text]  
  • Diaz, M. R., Fell, J. W. (2005). Use of a Suspension Array for Rapid Identification of the Varieties and Genotypes of the Cryptococcus neoformans Species Complex. J. Clin. Microbiol. 43: 3662-3672 [Abstract] [Full Text]  
  • Amjad, M., Kfoury, N., Cha, R., Mobarak, R. (2004). Quantification and assessment of viability of Cryptococcus neoformans by LightCycler amplification of capsule gene mRNA. J Med Microbiol 53: 1201-1206 [Abstract] [Full Text]  
  • Yeo, S. F., Wong, B. (2002). Current Status of Nonculture Methods for Diagnosis of Invasive Fungal Infections. Clin. Microbiol. Rev. 15: 465-484 [Abstract] [Full Text]  
  • Bialek, R., Weiss, M., Bekure-Nemariam, K., Najvar, L. K., Alberdi, M. B., Graybill, J. R., Reischl, U. (2002). Detection of Cryptococcus neoformans DNA in Tissue Samples by Nested and Real-Time PCR Assays. CVI 9: 461-469 [Abstract] [Full Text]  
  • Lindsley, M. D., Hurst, S. F., Iqbal, N. J., Morrison, C. J. (2001). Rapid Identification of Dimorphic and Yeast-Like Fungal Pathogens Using Specific DNA Probes. J. Clin. Microbiol. 39: 3505-3511 [Abstract] [Full Text]  
  • Martin, C., Roberts, D., van der Weide, M., Rossau, R., Jannes, G., Smith, T., Maher, M. (2000). Development of a PCR-Based Line Probe Assay for Identification of Fungal Pathogens. J. Clin. Microbiol. 38: 3735-3742 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»