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Journal of Clinical Microbiology, December 1998, p. 3532-3539, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Clonal Distribution of Methicillin-Resistant Staphylococcus aureus in Poland

T. Leski,1,2 D. Oliveira,1 K. Trzcinski,2 I. Santos Sanches,1,3 M. Aires de Sousa,1 W. Hryniewicz,2 and H. de Lencastre1,3,4,*

Molecular Genetics Unit, Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras,1 and Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Monte da Caparica,3 Portugal; Sera and Vaccines Central Research Laboratory, Warsaw, Poland2; and Laboratory of Microbiology, The Rockefeller University, New York, New York4

Received 15 June 1998/Returned for modification 8 July 1998/Accepted 2 September 1998

We report on a study of 158 methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates obtained from 1990 to 1996 in 18 different hospitals in Poland. All isolates were recovered from infection and carriage sites of patients, carriage sites of health care personnel, and hospital environment samples. Fifty-seven MRSA strains described here were studied previously and these were divided into two different clusters according to the degree of heterogeneity of methicillin resistance expression. The aim of this study was to extend the correlation between the two clusters and identify the clonal identities among all isolates by a combination of different methodologies: (i) analysis of mecA polymorphs and Tn554 insertion patterns and (ii) determination of pulsed-field gel electrophoresis patterns of chromosomal SmaI digests. Ninety-seven of 158 strains showed a heterogeneous expression of resistance to methicillin. Among these, 75 (77.3%) were ClaI-mecA type I, ClaI-Tn554 type NH (NH, no homology with transposon Tn554), and pulsed-field gel electrophoresis (PFGE) pattern A (I::NH::A); 10 isolates were III::B::M (10.3%); and the remaining clones included a few or single isolates. The isolates with homogeneous expression of resistance to methicillin (n = 61) were predominantly ClaI-mecA type III (49 of 61 [80.3%]) but had great variability in their ClaI-Tn554 and PFGE patterns. This study confirmed the existence of two main clusters of MRSA in Poland.

* Corresponding author. Mailing address: The Rockefeller University, 1230 York Ave., New York, NY 10021. Phone: (212) 327-8278. Fax: (212) 327-8688. E-mail: lencash{at}rockvax.rockefeller.edu.

Journal of Clinical Microbiology, December 1998, p. 3532-3539, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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