This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pasloske, B. L.
Right arrow Articles by DuBois, D. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pasloske, B. L.
Right arrow Articles by DuBois, D. B.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 1998, p. 3590-3594, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Armored RNA Technology for Production of Ribonuclease-Resistant Viral RNA Controls and Standards

Brittan L. Pasloske,1,* Cindy R. Walkerpeach,2 R. Dawn Obermoeller,1 Matthew Winkler,1 and Dwight B. DuBois2

Ambion, Inc.,1 and Cenetron Diagnostics,2 Austin, Texas 78744

Received 15 June 1998/Returned for modification 24 August 1998/Accepted 18 September 1998

The widespread use of sensitive assays for the detection of viral and cellular RNA sequences has created a need for stable, well-characterized controls and standards. We describe the development of a versatile, novel system for creating RNase-resistant RNA. "Armored RNA" is a complex of MS2 bacteriophage coat protein and RNA produced in Escherichia coli by the induction of an expression plasmid that encodes the coat protein and an RNA standard sequence. The RNA sequences are completely protected from RNase digestion within the bacteriophage-like complexes. As a prototype, a 172-base consensus sequence from a portion of the human immunodeficiency virus type 1 (HIV-1) gag gene was synthesized and cloned into the packaging vector used to produce the bacteriophage-like particles. After production and purification, the resulting HIV-1 Armored RNA particles were shown to be resistant to degradation in human plasma and produced reproducible results in the Amplicor HIV-1 Monitor assay for 180 days when stored at -20°C or for 60 days at 4°C. Additionally, Armored RNA preparations are homogeneous and noninfectious.

* Corresponding author. Mailing address: Ambion, Inc., 2130 Woodward St., #200, Austin, TX 78744-1832. Phone: (512) 651-0200, ext. 6120. Fax: (512) 651-0201. E-mail: bpasloske{at}ambion.com.

Journal of Clinical Microbiology, December 1998, p. 3590-3594, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Zhan, S., Li, J., Xu, R., Wang, L., Zhang, K., Zhang, R. (2009). Armored Long RNA Controls or Standards for Branched DNA Assay for Detection of Human Immunodeficiency Virus Type 1. J. Clin. Microbiol. 47: 2571-2576 [Abstract] [Full Text]  
  • Bull, M. E., Learn, G. H., McElhone, S., Hitti, J., Lockhart, D., Holte, S., Dragavon, J., Coombs, R. W., Mullins, J. I., Frenkel, L. M. (2009). Monotypic Human Immunodeficiency Virus Type 1 Genotypes across the Uterine Cervix and in Blood Suggest Proliferation of Cells with Provirus. J. Virol. 83: 6020-6028 [Abstract] [Full Text]  
  • Picard, F. J., Gagnon, M., Bernier, M. R., Parham, N. J., Bastien, M., Boissinot, M., Peytavi, R., Bergeron, M. G. (2009). Internal Control for Nucleic Acid Testing Based on the Use of Purified Bacillus atrophaeus subsp. globigii Spores. J. Clin. Microbiol. 47: 751-757 [Abstract] [Full Text]  
  • Wei, Y., Yang, C., Wei, B., Huang, J., Wang, L., Meng, S., Zhang, R., Li, J. (2008). RNase-Resistant Virus-Like Particles Containing Long Chimeric RNA Sequences Produced by Two-Plasmid Coexpression System. J. Clin. Microbiol. 46: 1734-1740 [Abstract] [Full Text]  
  • Doleshal, M., Magotra, A. A., Choudhury, B., Cannon, B. D., Labourier, E., Szafranska, A. E. (2008). Evaluation and Validation of Total RNA Extraction Methods for MicroRNA Expression Analyses in Formalin-Fixed, Paraffin-Embedded Tissues. J. Mol. Diagn. 10: 203-211 [Abstract] [Full Text]  
  • Yu, X.-F., Pan, J.-C., Ye, R., Xiang, H.-Q., Kou, Y., Huang, Z.-C. (2008). Preparation of Armored RNA as a Control for Multiplex Real-Time Reverse Transcription-PCR Detection of Influenza Virus and Severe Acute Respiratory Syndrome Coronavirus. J. Clin. Microbiol. 46: 837-841 [Abstract] [Full Text]  
  • Hewitt, J., Bell, D., Simmons, G. C., Rivera-Aban, M., Wolf, S., Greening, G. E. (2007). Gastroenteritis Outbreak Caused by Waterborne Norovirus at a New Zealand Ski Resort. Appl. Environ. Microbiol. 73: 7853-7857 [Abstract] [Full Text]  
  • Villanova, G. V., Gardiol, D., Taborda, M. A., Reggiardo, V., Tanno, H., Rivadeneira, E. D., Perez, G. R., Giri, A. A. (2007). Strategic Approach To Produce Low-Cost, Efficient, and Stable Competitive Internal Controls for Detection of RNA Viruses by Use of Reverse Transcription-PCR. J. Clin. Microbiol. 45: 3555-3563 [Abstract] [Full Text]  
  • Gotsch, A., Schubert, A., Bombis, A., Wiedmann, M., Zauke, M., Schorling, S. (2007). Nuclease-Resistant Single-Stranded DNA Controls for Nucleic Acid Amplification Assays. J. Clin. Microbiol. 45: 2570-2574 [Abstract] [Full Text]  
  • Cheng, Y., Niu, J., Zhang, Y., Huang, J., Li, Q. (2006). Preparation of His-Tagged Armored RNA Phage Particles as a Control for Real-Time Reverse Transcription-PCR Detection of Severe Acute Respiratory Syndrome Coronavirus.. J. Clin. Microbiol. 44: 3557-3561 [Abstract] [Full Text]  
  • Das, A., Spackman, E., Senne, D., Pedersen, J., Suarez, D. L. (2006). Development of an Internal Positive Control for Rapid Diagnosis of Avian Influenza Virus Infections by Real-Time Reverse Transcription-PCR with Lyophilized Reagents.. J. Clin. Microbiol. 44: 3065-3073 [Abstract] [Full Text]  
  • Huang, Q., Cheng, Y., Guo, Q., Li, Q. (2006). Preparation of a chimeric armored RNA as a versatile calibrator for multiple virus assays.. Clin. Chem. 52: 1446-1448 [Full Text]  
  • Hietala, S. K., Crossley, B. M. (2006). Armored RNA as Virus Surrogate in a Real-Time Reverse Transcriptase PCR Assay Proficiency Panel. J. Clin. Microbiol. 44: 67-70 [Abstract] [Full Text]  
  • Antonishyn, N. A., Ast, V. M., McDonald, R. R., Chaudhary, R. K., Lin, L., Andonov, A. P., Horsman, G. B. (2005). Rapid Genotyping of Hepatitis C Virus by Primer-Specific Extension Analysis. J. Clin. Microbiol. 43: 5158-5163 [Abstract] [Full Text]  
  • Dreier, J., Stormer, M., Kleesiek, K. (2005). Use of Bacteriophage MS2 as an Internal Control in Viral Reverse Transcription-PCR Assays. J. Clin. Microbiol. 43: 4551-4557 [Abstract] [Full Text]  
  • WalkerPeach, C. R., Pasloske, B. L. (2004). DNA Bacteriophage as Controls for Clinical Viral Testing. Clin. Chem. 50: 1970-1971 [Full Text]  
  • Stocher, M., Berg, J. (2004). Internal Control DNA for PCR Assays Introduced into Lambda Phage Particles Exhibits Nuclease Resistance. Clin. Chem. 50: 2163-2166 [Full Text]  
  • Beld, M., Minnaar, R., Weel, J., Sol, C., Damen, M., van der Avoort, H., Wertheim-van Dillen, P., van Breda, A., Boom, R. (2004). Highly Sensitive Assay for Detection of Enterovirus in Clinical Specimens by Reverse Transcription-PCR with an Armored RNA Internal Control. J. Clin. Microbiol. 42: 3059-3064 [Abstract] [Full Text]  
  • Nolte, F. S. (2004). Novel Internal Controls For Real-Time PCR Assays. Clin. Chem. 50: 801-802 [Full Text]  
  • Bressler, A. M., Nolte, F. S. (2004). Preclinical Evaluation of Two Real-Time, Reverse Transcription-PCR Assays for Detection of the Severe Acute Respiratory Syndrome Coronavirus. J. Clin. Microbiol. 42: 987-991 [Abstract] [Full Text]  
  • Dingle, K. E., Crook, D., Jeffery, K. (2004). Stable and Noncompetitive RNA Internal Control for Routine Clinical Diagnostic Reverse Transcription-PCR. J. Clin. Microbiol. 42: 1003-1011 [Abstract] [Full Text]  
  • Koenig, G. L. (2003). Viability of and Plasmid Retention in Frozen Recombinant Escherichia coli over Time: a Ten-Year Prospective Study. Appl. Environ. Microbiol. 69: 6605-6609 [Abstract] [Full Text]  
  • Kleiboeker, S. B. (2003). Applications of Competitor RNA in Diagnostic Reverse Transcription-PCR. J. Clin. Microbiol. 41: 2055-2061 [Abstract] [Full Text]  
  • Drosten, C., Seifried, E., Roth, W. K. (2001). TaqMan 5'-Nuclease Human Immunodeficiency Virus Type 1 PCR Assay with Phage-Packaged Competitive Internal Control for High-Throughput Blood Donor Screening. J. Clin. Microbiol. 39: 4302-4308 [Abstract] [Full Text]  
  • Verstrepen, W. A., Kuhn, S., Kockx, M. M., Van De Vyvere, M. E., Mertens, A. H. (2001). Rapid Detection of Enterovirus RNA in Cerebrospinal Fluid Specimens with a Novel Single-Tube Real-Time Reverse Transcription-PCR Assay. J. Clin. Microbiol. 39: 4093-4096 [Abstract] [Full Text]  
  • Callahan, J. D., Wu, S.-J. L., Dion-Schultz, A., Mangold, B. E., Peruski, L. F., Watts, D. M., Porter, K. R., Murphy, G. R., Suharyono, W., King, C.-C., Hayes, C. G., Temenak, J. J. (2001). Development and Evaluation of Serotype- and Group-Specific Fluorogenic Reverse Transcriptase PCR (TaqMan) Assays for Dengue Virus. J. Clin. Microbiol. 39: 4119-4124 [Abstract] [Full Text]  
  • Cartwright, C. P. (1999). Synthetic Viral Particles Promise To Be Valuable in the Standardization of Molecular Diagnostic Assays for Hepatitis C Virus. Clin. Chem. 45: 2057-2059 [Full Text]  
  • WalkerPeach, C. R., Winkler, M., DuBois, D. B., Pasloske, B. L. (1999). Ribonuclease-resistant RNA Controls (Armored RNA) for Reverse Transcription-PCR, Branched DNA, and Genotyping Assays for Hepatitis C Virus. Clin. Chem. 45: 2079-2085 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»