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Journal of Clinical Microbiology, December 1998, p. 3689-3690, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Rapid PCR Detection of Helicobacter pylori-Associated Virulence and Resistance Genes Directly from Gastric Biopsy Material

Britta Björkholm,1,2 Ragnar Befrits,1 Bernhard Jaup,3 and Lars Engstrand2,4,*

Department of Medicine, Karolinska Institute,1 and Swedish Institute for Infectious Disease Control,4 Stockholm, Department of Clinical Microbiology, University Hospital, Uppsala,2 and Department of Medicine, Lundby Hospital, Göteborg,3 Sweden

Received 21 April 1998/Returned for modification 26 June 1998/Accepted 4 September 1998

We have developed a PCR-based method to detect macrolide resistance and the virulence gene cagA in Helicobacter pylori within 24 h, thereby improving the lengthy process of culture-based approaches. Total DNA was prepared directly from stomach biopsy specimens. The procedure proved to be rapid and reliable and could be utilized for diagnostic purposes.

* Corresponding author. Mailing address: Swedish Institute for Infectious Disease Control, 171 82 Solna, Sweden. Phone: 46-8-457 24 15. Fax: 46-8-30 17 97. E-mail: Lars.Engstrand{at}smi.ki.se.

Journal of Clinical Microbiology, December 1998, p. 3689-3690, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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