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Journal of Clinical Microbiology, December 1998, p. 3737-3739, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Specific Antibodies in Saliva during Dengue Infection

Andrea J. Cuzzubbo,1 David W. Vaughn,2,dagger Ananda Nisalak,2 Saroj Suntayakorn,3 John Aaskov,4 and Peter L. Devine1,*

PanBio Pty Ltd., Windsor, Queensland,1 and Department of Immunology, School of Life Sciences, Queensland University of Technology, Brisbane,4 Australia, and Department of Virology, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok,2 and Department of Pediatrics, Kamphaeng Phet Provincial Hospital, Kamphaeng Phet,3 Thailand

Received 18 June 1998/Returned for modification 8 July 1998/Accepted 1 September 1998

Saliva was collected prospectively from patients presenting with suspected dengue infection 4 to 8 days after the onset of symptoms and assayed by a commercial dengue immunoglobulin M (IgM) and IgG capture enzyme-linked immunosorbent assay (ELISA) (PanBio Dengue Duo ELISA). Laboratory diagnosis was based on virus isolation and on hemagglutination inhibition (HAI) assay and an in-house IgM and IgG capture ELISA. With a positive result defined as either salivary IgM or IgG levels above the cutoff value, an overall sensitivity of 92% was obtained for both primary- and secondary-dengue patients (22 of 24), while no patients with non-flavivirus infections (n = 11) and no healthy laboratory donors (n = 17) showed elevation of salivary antidengue antibody (100% specificity). Salivary IgG levels correlated well with serum HAI titer (r = 0.78), and salivary IgG levels could be used to distinguish between primary- and secondary-dengue virus infections.

* Corresponding author. Mailing address: PanBio Pty Ltd., 116 Lutwyche Rd., Windsor, Queensland 4030, Australia. Phone: 61-7-33571177. Fax: 61-7-33571222. E-mail: peter_devine{at}panbio.com.au.

dagger Present address: Department of Virus Diseases, Walter Reed Army Institute of Research, Washington, D.C.

Journal of Clinical Microbiology, December 1998, p. 3737-3739, Vol. 36, No. 12
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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