This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Haque, R.
Right arrow Articles by Petri, W. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Haque, R.
Right arrow Articles by Petri, W. A., Jr.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 1998, p. 449-452, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of PCR, Isoenzyme Analysis, and Antigen Detection for Diagnosis of Entamoeba histolytica Infection

Rashidul Haque,1 I. K. M. Ali,1 S. Akther,1 and William A. Petri Jr.2,*

International Centre for Diarrheal Disease Research, Dhaka, Bangladesh,1 and Departments of Medicine, Microbiology, and Pathology, University of Virginia, Charlottesville, Virginia2

Received 19 August 1997/Returned for modification 21 October 1997/Accepted 4 November 1997

The diagnosis of amebiasis by microscopic identification of the parasite in stool is insensitive and unable to distinguish the invasive parasite Entamoeba histolytica from the commensal parasite E. dispar. In this study, we have tested a PCR technique for the detection of E. histolytica and compared it with isoenzyme analysis and the TechLab E. histolytica-specific antigen detection test. The nested-PCR test we used is based on amplification of the small subunit rRNA gene of E. histolytica and E. dispar followed by restriction digest analysis of the PCR product. Single stool samples were obtained from 98 patients from Dhaka, Bangladesh, with diarrhea: 88 patients diagnosed by microscopy and/or culture with E. histolytica and/or E. dispar infection and 10 patients without infection. Isoenzyme analysis identified 53 of the infections as E. histolytica and 28 as E. dispar. PCR and isoenzyme identification of E. histolytica agreed in 96% (51 of 53) of amebic cultures. PCR for E. histolytica was negative in all 10 samples that were negative for E. histolytica by isoenzyme and antigen detection. PCR and antigen detection had comparable sensitivities when performed directly on fresh stool specimens, identifying 87% (46 of 53) and 85% (45 of 53), respectively, of E. histolytica infections identified by isoenzyme analysis. The correlation of results by antigen detection and PCR for identification of E. histolytica in stool was 93% (45 of 48 cases). Mixed infections with E. histolytica and E. dispar were detected by PCR in 14% (12 of 88) of cases. In conclusion, all three techniques for specific identification of E. histolytica in fresh stool showed excellent correlation. Only the TechLab E. histolytica antigen detection test was both rapid and technically simple.

* Corresponding author. Mailing address: Room 2115 MR4 Building, University of Virginia Health Sciences Center, Charlottesville, VA 22908. Phone: (804) 924-5621. Fax: (804) 924-0075. E-mail: wap3g{at}virginia.edu.

Journal of Clinical Microbiology, February 1998, p. 449-452, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • de Saussure, P. P. H. (2009). Review: Management of the returning traveler with diarrhea. Therapeutic Advances in Gastroenterology 2: 367-375 [Abstract]  
  • Liang, S.-Y., Chan, Y.-H., Hsia, K.-T., Lee, J.-L., Kuo, M.-C., Hwa, K.-Y., Chan, C.-W., Chiang, T.-Y., Chen, J.-S., Wu, F.-T., Ji, D.-D. (2009). Development of Loop-Mediated Isothermal Amplification Assay for Detection of Entamoeba histolytica. J. Clin. Microbiol. 47: 1892-1895 [Abstract] [Full Text]  
  • Buss, S., Kabir, M., Petri, W. A. Jr., Haque, R. (2008). Comparison of Two Immunoassays for Detection of Entamoeba histolytica. J. Clin. Microbiol. 46: 2778-2779 [Abstract] [Full Text]  
  • Stark, D., van Hal, S., Fotedar, R., Butcher, A., Marriott, D., Ellis, J., Harkness, J. (2008). Comparison of Stool Antigen Detection Kits to PCR for Diagnosis of Amebiasis. J. Clin. Microbiol. 46: 1678-1681 [Abstract] [Full Text]  
  • Fotedar, R., Stark, D., Beebe, N., Marriott, D., Ellis, J., Harkness, J. (2007). Laboratory Diagnostic Techniques for Entamoeba Species. Clin. Microbiol. Rev. 20: 511-532 [Abstract] [Full Text]  
  • HAQUE, R., ROY, S., SIDDIQUE, A., MONDAL, U., RAHMAN, S. M. M., MONDAL, D., HOUPT, E., PETRI, W. A. JR (2007). MULTIPLEX REAL-TIME PCR ASSAY FOR DETECTION OF ENTAMOEBA HISTOLYTICA, GIARDIA INTESTINALIS, AND CRYPTOSPORIDIUM SPP.. Am J Trop Med Hyg 76: 713-717 [Abstract] [Full Text]  
  • TANYUKSEL, M., ULUKANLIGIL, M., GUCLU, Z., ARAZ, E., KORU, O., PETRI, W. A. JR. (2007). TWO CASES OF RARELY RECOGNIZED INFECTION WITH ENTAMOEBA MOSHKOVSKII. Am J Trop Med Hyg 76: 723-724 [Abstract] [Full Text]  
  • STARK, D., FOTEDAR, R., VAN HAL, S., BEEBE, N., MARRIOTT, D., ELLIS, J. T., HARKNESS, J. (2007). PREVALENCE OF ENTERIC PROTOZOA IN HUMAN IMMUNODEFICIENCY VIRUS (HIV)-POSITIVE AND HIV-NEGATIVE MEN WHO HAVE SEX WITH MEN FROM SYDNEY, AUSTRALIA. Am J Trop Med Hyg 76: 549-552 [Abstract] [Full Text]  
  • Leo, M., Haque, R., Kabir, M., Roy, S., Lahlou, R. M., Mondal, D., Tannich, E., Petri, W. A. Jr. (2006). Evaluation of Entamoeba histolytica Antigen and Antibody Point-of-Care Tests for the Rapid Diagnosis of Amebiasis. J. Clin. Microbiol. 44: 4569-4571 [Abstract] [Full Text]  
  • Hamzah, Z., Petmitr, S., Mungthin, M., Leelayoova, S., Chavalitshewinkoon-Petmitr, P. (2006). Differential Detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii by a Single-Round PCR Assay.. J. Clin. Microbiol. 44: 3196-3200 [Abstract] [Full Text]  
  • SAMIE, A., OBI, L. C., BESSONG, P. O., STROUP, S., HOUPT, E., GUERRANT, R. L. (2006). Prevalence and species distribution of e. Histolytica and e. Dispar in the venda region, limpopo, South Africa.. Am J Trop Med Hyg 75: 565-571 [Abstract] [Full Text]  
  • Ali, I. K. M., Zaki, M., Clark, C. G. (2005). Use of PCR Amplification of tRNA Gene-Linked Short Tandem Repeats for Genotyping Entamoeba histolytica. J. Clin. Microbiol. 43: 5842-5847 [Abstract] [Full Text]  
  • Qvarnstrom, Y., James, C., Xayavong, M., Holloway, B. P., Visvesvara, G. S., Sriram, R., da Silva, A. J. (2005). Comparison of Real-Time PCR Protocols for Differential Laboratory Diagnosis of Amebiasis. J. Clin. Microbiol. 43: 5491-5497 [Abstract] [Full Text]  
  • van Doorn, H. R., Hofwegen, H., Koelewijn, R., Gilis, H., Peek, R., Wetsteyn, J. C. F. M., van Genderen, P. J. J., Vervoort, T., van Gool, T. (2005). Use of Rapid Dipstick and Latex Agglutination Tests and Enzyme-Linked Immunosorbent Assay for Serodiagnosis of Amebic Liver Abscess, Amebic Colitis, and Entamoeba histolytica Cyst Passage. J. Clin. Microbiol. 43: 4801-4806 [Abstract] [Full Text]  
  • Bhattacharya, D., Haque, R., Singh, U. (2005). Coding and Noncoding Genomic Regions of Entamoeba histolytica Have Significantly Different Rates of Sequence Polymorphisms: Implications for Epidemiological Studies. J. Clin. Microbiol. 43: 4815-4819 [Abstract] [Full Text]  
  • Roy, S., Kabir, M., Mondal, D., Ali, I. K. M., Petri, W. A. Jr., Haque, R. (2005). Real-Time-PCR Assay for Diagnosis of Entamoeba histolytica Infection. J. Clin. Microbiol. 43: 2168-2172 [Abstract] [Full Text]  
  • Furrows, S J, Moody, A H, Chiodini, P L (2004). Comparison of PCR and antigen detection methods for diagnosis of Entamoeba histolytica infection. J. Clin. Pathol. 57: 1264-1266 [Abstract] [Full Text]  
  • Tanyuksel, M., Petri, W. A. Jr. (2003). Laboratory Diagnosis of Amebiasis. Clin. Microbiol. Rev. 16: 713-729 [Abstract] [Full Text]  
  • Haque, R., Huston, C. D., Hughes, M., Houpt, E., Petri, W. A. Jr. (2003). Amebiasis. NEJM 348: 1565-1573 [Full Text]  
  • Gonin, P., Trudel, L. (2003). Detection and Differentiation of Entamoeba histolytica and Entamoeba dispar Isolates in Clinical Samples by PCR and Enzyme-Linked Immunosorbent Assay. J. Clin. Microbiol. 41: 237-241 [Abstract] [Full Text]  
  • Zaki, M., Meelu, P., Sun, W., Clark, C. G. (2002). Simultaneous Differentiation and Typing of Entamoeba histolytica and Entamoeba dispar. J. Clin. Microbiol. 40: 1271-1276 [Abstract] [Full Text]  
  • Yau, Y. C. W., Crandall, I., Kain, K. C. (2001). Development of Monoclonal Antibodies Which Specifically Recognize Entamoeba histolytica in Preserved Stool Samples. J. Clin. Microbiol. 39: 716-719 [Abstract] [Full Text]  
  • Sharp, S. E., Suarez, C. A., Duran, Y., Poppiti, R. J. (2001). Evaluation of the Triage Micro Parasite Panel for Detection of Giardia lamblia, Entamoeba histolytica/Entamoeba dispar, and Cryptosporidium parvum in Patient Stool Specimens. J. Clin. Microbiol. 39: 332-334 [Abstract] [Full Text]  
  • Haque, R., Mollah, N. U., Ali, I. K. M., Alam, K., Eubanks, A., Lyerly, D., Petri, W. A. Jr. (2000). Diagnosis of Amebic Liver Abscess and Intestinal Infection with the TechLab Entamoeba histolytica II Antigen Detection and Antibody Tests. J. Clin. Microbiol. 38: 3235-3239 [Abstract] [Full Text]  
  • Garcia, L. S., Shimizu, R. Y., Bernard, C. N. (2000). Detection of Giardia lamblia, Entamoeba histolytica/Entamoeba dispar, and Cryptosporidium parvum Antigens in Human Fecal Specimens Using the Triage Parasite Panel Enzyme Immunoassay. J. Clin. Microbiol. 38: 3337-3340 [Abstract] [Full Text]  
  • Que, X., Reed, S. L. (2000). Cysteine Proteinases and the Pathogenesis of Amebiasis. Clin. Microbiol. Rev. 13: 196-206 [Abstract] [Full Text]  
  • Espinosa-Cantellano, M., Martinez-Palomo, A. (2000). Pathogenesis of Intestinal Amebiasis: From Molecules to Disease. Clin. Microbiol. Rev. 13: 318-331 [Abstract] [Full Text]  
  • Pillai, D. R., Kain, K. C. (1999). Immunochromatographic Strip-Based Detection of Entamoeba histolytica-E. dispar and Giardia lamblia Coproantigen. J. Clin. Microbiol. 37: 3017-3019 [Abstract] [Full Text]  
  • Braga, L. L., Mendonca, Y., Paiva, C. A., Sales, A., Cavalcante, A. L. M., Mann, B. J. (1998). Seropositivity for and Intestinal Colonization with Entamoeba histolytica and Entamoeba dispar in Individuals in Northeastern Brazil. J. Clin. Microbiol. 36: 3044-3045 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»