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Journal of Clinical Microbiology, February 1998, p. 569-572, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Oligonucleotide Ligation Assay for Detecting Mutations in the Human Immunodeficiency Virus Type 1 pol Gene That Are Associated with Resistance to Zidovudine, Didanosine, and Lamivudine

Reva E. Edelstein,1 Deborah A. Nickerson,2 Vincent O. Tobe,2 Laura A. Manns-Arcuino,1 and Lisa M. Frenkel1,*

Division of Infectious Diseases, Department of Pediatrics,1 and Department of Molecular Biotechnology,2 University of Washington, Seattle, Washington

Received 10 June 1997/Returned for modification 18 September 1997/Accepted 3 November 1997

This report describes the detection of mutations in the pol gene of human immunodeficiency virus type 1 associated with resistance to zidovudine, didanosine, and lamivudine by genotyping by an oligonucleotide ligation assay specific codons in the pol gene amplified by PCR. Our studies demonstrate the sensitivity, simplicity, and specificity of this genotyping system.

* Corresponding author. Mailing address: 4800 Sand Point Way NE, CH-32, Seattle, WA 98105. Phone: (206) 526-2116. Fax: (206) 527-3890. E-mail: lfrenkel{at}u.washington.edu.

Journal of Clinical Microbiology, February 1998, p. 569-572, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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