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Journal of Clinical Microbiology, February 1998, p. 573-576, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

DNA Fingerprinting of Mycobacterium tuberculosis Complex Culture Isolates Collected in Brazil and Spotted onto Filter Paper

Marion Burger,1,2,3 Salmo Raskin,1 Sonia R. Brockelt,4 Beate Amthor,2 Heinrich K. Geiss,3 and Walter H. Haas2,*

GENETIKA, Laboratory of Genetics,1 and LACEN (Central Laboratory of the State of Paraná),4 Curitiba, Brazil, and Department of General Pediatrics, Children's Hospital,2 and Institute for Medical Microbiology and Hygiene,3 University of Heidelberg, Heidelberg, Germany

Received 2 September 1997/Returned for modification 18 October 1997/Accepted 8 November 1997

The usefulness of filter paper for preservation of bacterial cells was shown by mixed-linker DNA fingerprint analysis of Mycobacterium tuberculosis isolates from 77 Brazilian patients. DNA fingerprints of samples spotted onto filter paper and conventional culture material were identical. Thus, filter paper specimens analyzed by an amplification-based typing method provide a new resource for epidemiological studies of infectious diseases.

* Corresponding author. Mailing address: Department of General Pediatrics, University Children's Hospital, University of Heidelberg, Im Neuenheimer Feld 150, 69120 Heidelberg, Germany. Phone: 49 6221-568389. Fax: 49 6221-564388. E-mail: ic1{at}ix.urz.uni-heidelberg.de.

Journal of Clinical Microbiology, February 1998, p. 573-576, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.




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