This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chou, S.
Right arrow Articles by Kasatiya, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chou, S.
Right arrow Articles by Kasatiya, S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 1998, p. 577-579, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Use of Gas Chromatographic Fatty Acid and Mycolic Acid Cleavage Product Determination To Differentiate among Mycobacterium genavense, Mycobacterium fortuitum, Mycobacterium simiae, and Mycobacterium tuberculosis

S. Chou,1,* P. Chedore,2 and S. Kasatiya1,3

Public Health Laboratory, Ontario Ministry of Health, Ottawa, Ontario, Canada K1G 6C41; Public Health Laboratory, Ontario Ministry of Health, Etobicoke, Ontario, Canada M9P 3T12; and Department of Microbiology and Immunology, University of Ottawa, Ontario, Canada K1N 6N53

Received 24 February 1997/Returned for modification 23 April 1997/Accepted 18 November 1997

Three Mycobacterium genavense strains and three American Type Culture Collection reference strains each of Mycobacterium fortuitum, Mycobacterium simiae, and Mycobacterium tuberculosis were subcultured onto Mycobacteria 7H11 agar (Difco Laboratories, Detroit, Mich.) supplemented with mycobactin J (Allied Laboratories, Fayette, Mo.). After 4 weeks of incubation at 37°C in 10% CO2, the cultures were analyzed by gas-liquid chromatography (GLC) for their fatty acids and mycolic acid cleavage products. M. fortuitum was clearly differentiated from M. genavense by the presence of the specific marker 2-methyloctadecenoic acid in M. fortuitum and by the ratio of tetracosanoic acid to hexacosanoic acid. This ratio was <1 for M. genavense and >3 for M. fortuitum. M. fortuitum also contained docosanoic acid, which was not detected in M. genavense. M. genavense, M. simiae, and M. tuberculosis, which have similar GLC profiles, were also differentiated from each other by the presence of either cis-10-hexadecenoic acid or cis-11-hexadecenoic acid and by tetradecanoic acid content.

* Corresponding author. Mailing address: 2380 St. Laurent Blvd., Ottawa, Ontario K1G 6C4, Canada. Phone: (613) 736-6800. Fax: (613) 736-6820. E-mail: LSB_Ottawa{at}moh.gov.on.ca.

Journal of Clinical Microbiology, February 1998, p. 577-579, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • le Roes-Hill, M., Meyers, P. R. (2009). Streptomyces polyantibioticus sp. nov., isolated from the banks of a river. Int. J. Syst. Evol. Microbiol. 59: 1302-1309 [Abstract] [Full Text]  
  • Yam, W.-C., Yuen, K.-Y., Kam, S.-Y., Yiu, L.-S., Chan, K.-S., Leung, C.-C., Tam, C.-M., Ho, P.-O., Yew, W.-W., Seto, W.-H., Ho, P.-L. (2006). Diagnostic application of genotypic identification of mycobacteria. J Med Microbiol 55: 529-536 [Abstract] [Full Text]  
  • Cook, A. E., Roes, M. l., Meyers, P. R. (2005). Actinomadura napierensis sp. nov., isolated from soil in South Africa. Int. J. Syst. Evol. Microbiol. 55: 703-706 [Abstract] [Full Text]  
  • Phetsuksiri, B., Jackson, M., Scherman, H., McNeil, M., Besra, G. S., Baulard, A. R., Slayden, R. A., DeBarber, A. E., Barry, C. E. III, Baird, M. S., Crick, D. C., Brennan, P. J. (2003). Unique Mechanism of Action of the Thiourea Drug Isoxyl on Mycobacterium tuberculosis. J. Biol. Chem. 278: 53123-53130 [Abstract] [Full Text]  
  • Torkko, P., Katila, M.-L., Kontro, M. (2003). Gas-chromatographic lipid profiles in identification of currently known slowly growing environmental mycobacteria. J Med Microbiol 52: 315-323 [Abstract] [Full Text]  
  • Suomalainen, S., Koukila-Kahkola, P., Brander, E., Katila, M.-L., Piilonen, A., Paulin, L., Mattson, K. (2001). Pulmonary Infection Caused by an Unusual, Slowly Growing Nontuberculous Mycobacterium. J. Clin. Microbiol. 39: 2668-2671 [Abstract] [Full Text]  
  • Torkko, P., Suomalainen, S., Iivanainen, E., Suutari, M., Paulin, L., Rudbäck, E., Tortoli, E., Vincent, V., Mattila, R., Katila, M.-L. (2001). Characterization of Mycobacterium bohemicum Isolated from Human, Veterinary, and Environmental Sources. J. Clin. Microbiol. 39: 207-211 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»