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Journal of Clinical Microbiology, February 1998, p. 592-594, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of Eight Methods To Detect Vancomycin Resistance in Enterococci

Hubert P. Endtz,1,* Nicole Van Den Braak,1 Alex Van Belkum,1 Wil H. Goessens,1 Deborah Kreft,1 A. Barnard Stroebel,2 and Henri A. Verbrugh1

Erasmus University Medical Center Rotterdam1 and St. Clara Hospital,2 Rotterdam, The Netherlands

Received 21 August 1997/Returned for modification 10 October 1997/Accepted 13 November 1997

A collection of genetically unrelated vancomycin-resistant enterococci (VRE) including 50 vanA, 15 vanB, 50 vanC1, and 30 vanC2 VRE were used to evaluate the accuracy of eight currently available susceptibility test methods (agar dilution, disk diffusion, E-test, agar screen plate, Vitek GPS-TA and GPS-101, and MicroScan overnight and rapid panels). vanA VRE were detected by all methods. vanB VRE were often not detected by Vitek GPS-TA and MicroScan rapid (sensitivities, 47 and 53%, respectively), though the new Vitek GPS-101 was found to be a significant improvement. E-test and the agar screen were the only two methods detecting all VRE, including the vanC1/C2 VRE.

* Corresponding author. Mailing address: Department of Medical Microbiology & Infectious Diseases, Erasmus University Medical Center Rotterdam, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. Phone: (31)10 4635820. Fax: (31)10 4633875. E-mail: ENDTZ{at}BACL.AZR.NL.

Journal of Clinical Microbiology, February 1998, p. 592-594, Vol. 36, No. 2
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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