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Journal of Clinical Microbiology, March 1998, p. 748-751, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Standardized BACTEC Method To Measure Clarithromycin Susceptibility of Mycobacterium genavense

La Donna C. Carlson,1 Carolyn K. Wallis,1 and Marie B. Coyle1,2,*

Department of Laboratory Medicine, Harborview Medical Center, University of Washington, Seattle, Washington 98104,1 and Department of Microbiology, University of Washington, Seattle, Washington 981952

Received 1 July 1997/Returned for modification 6 September 1997/Accepted 26 November 1997

A standardized clarithromycin susceptibility test for Mycobacterium genavense is reported. The BACTEC radiometric broth dilution test method recommended for Mycobacterium avium complex was modified to develop a reliable and reproducible procedure. Test development involved optimization of medium pH and inoculum densities for antibiotic vials as well as growth control vials. MIC control organisms included Mycobacterium simiae, Mycobacterium avium, and Mycobacterium xenopi. Growth control vials required two to three inoculum dilutions, which varied for each species. Clarithromycin MICs and MBCs for 12 isolates and 1 colonial variant of M. genavense ranged from <= 0.06 to 0.25 µg/ml.

* Corresponding author. Mailing address: Department of Laboratory Medicine, Harborview Medical Center, Box 359743, 325 9th Ave., Seattle, WA 98104. Phone: (206) 731-3311. Fax: (206) 731-3930. E-mail: coyle{at}mail.labmed.washington.edu.

Journal of Clinical Microbiology, March 1998, p. 748-751, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.




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