This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Damond, F.
Right arrow Articles by Simon, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Damond, F.
Right arrow Articles by Simon, F.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 1998, p. 809-811, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Highly Sensitive Method for Amplification of Human Immunodeficiency Virus Type 2 DNA

Florence Damond,1,* Ibtissam Loussert-Ajaka,1 Cristian Apetrei,1,2 Diane Descamps,1 Sandrine Souquière,1 Annie Leprêtre,3 Sophie Matheron,3 Françoise Brun-Vézinet,1 and François Simon1

Laboratoire de Virologie1 and Service des Maladies Infectieuses et Tropicales,3 Hôpital Bichat-Claude Bernard, 75018 Paris, France, and Virus Laboratory, Microbiology Department, School of Medicine, "Gr. T. Popa" University, 6600 Iasi, Romania2

Received 30 July 1997/Returned for modification 27 October 1997/Accepted 10 December 1997

We evaluated a new human immunodeficiency virus type 2 (HIV-2) DNA amplification strategy based on peripheral blood mononuclear cell long PCR (XL PCR) followed by nested PCR amplification. The primers used were located in the highly conserved long terminal repeat and in the pol regions of the genome. Five primer pairs corresponding to different regions of the HIV-2 env gene were used in the nested step. Samples from 42 patients were tested, which yielded positive amplification with at least two primer pairs in 40 (95%) samples. A primer pair (EB2/EB5) located on the V3 region succeeded in amplifying proviral DNA in 40 samples.

* Corresponding author. Mailing address: Laboratoire de Virologie, Hôpital Bichat-Claude Bernard, 46 Rue Henri Huchard, 75018 Paris, France. Phone: 33/1 40 25 88 94. Fax: 33/1 46 27 02 08. E-mail: francois.simon{at}bch.ap-hop-paris.fr.

Journal of Clinical Microbiology, March 1998, p. 809-811, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Damond, F., Gueudin, M., Pueyo, S., Farfara, I., Robertson, D. L., Descamps, D., Chene, G., Matheron, S., Campa, P., Brun-Vezinet, F., Simon, F. (2002). Plasma RNA Viral Load in Human Immunodeficiency Virus Type 2 Subtype A and Subtype B Infections. J. Clin. Microbiol. 40: 3654-3659 [Abstract] [Full Text]  
  • Damond, F., Descamps, D., Farfara, I., Telles, J. N., Puyeo, S., Campa, P., Lepretre, A., Matheron, S., Brun-Vezinet, F., Simon, F. (2001). Quantification of Proviral Load of Human Immunodeficiency Virus Type 2 Subtypes A and B Using Real-Time PCR. J. Clin. Microbiol. 39: 4264-4268 [Abstract] [Full Text]  
  • Plantier, J.-C., Damond, F., Souquieres, S., Brun-Vezinet, F., Simon, F., Barin, F. (2001). V3 Serological Subtyping of Human Immunodeficiency Virus Type 2 Infection Is Not Relevant. J. Clin. Microbiol. 39: 3803-3807 [Abstract] [Full Text]  
  • Kannangai, R., Ramalingam, S., Prakash, K. J., Abraham, O. C., George, R., Castillo, R. C., Schwartz, D. H., Jesudason, M. V., Sridharan, G. (2000). Molecular Confirmation of Human Immunodeficiency Virus (HIV) Type 2 in HIV-Seropositive Subjects in South India. CVI 7: 987-989 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»