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Journal of Clinical Microbiology, March 1998, p. 833-834, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Optochin Revisited: Defining the Optimal Type of Blood Agar for Presumptive Identification of Streptococcus pneumoniae

M. A. Gardam and M. A. Miller*

Sir Mortimer B. Davis-Jewish General Hospital, McGill University, Montreal, Canada

Received 10 July 1997/Returned for modification 14 October 1997/Accepted 4 December 1997

To determine the optimal media for optochin susceptibility testing of Streptococcus pneumoniae, we measured inhibition zones for 72 S. pneumoniae and 22 Streptococcus viridans isolates on three blood-containing media. Because 15.3, 0, and 22.2% of S. pneumoniae organisms were misidentified on Columbia agar, Trypticase soy agar (TSA), and Mueller-Hinton agar, respectively, each containing sheep blood, we recommend that TSA-sheep blood agar be used.

* Corresponding author. Mailing address: Department of Microbiology, SMBD-Jewish General Hospital, 3755 Cote-Ste-Catherine, Montreal, QC, Canada, H3T 1E2. Phone: (514) 340-8294. Fax: (514) 340-7546. E-mail: mmiller{at}lab.jgh.mcgill.ca.

Journal of Clinical Microbiology, March 1998, p. 833-834, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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