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Journal of Clinical Microbiology, March 1998, p. 835-839, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Intra-Assay Performance Characteristics of Five Assays for Quantification of Human Immunodeficiency Virus Type 1 RNA in Plasma

Hsiang Ju Lin,1 Louise Pedneault,2,dagger and F. Blaine Hollinger1,*

Division of Molecular Virology, Baylor College of Medicine, Houston, Texas,1 and Antiviral Clinical Research Department, Bristol-Myers Squibb Pharmaceutical Research Institute, Wallingford, Connecticut2

Received 23 June 1997/Returned for modification 7 November 1997/Accepted 1 December 1997

Three kits (Roche AMPLICOR human immunodeficiency virus type 1 [HIV-1] Monitor, Chiron enhanced-sensitivity bDNA, and Organon Teknika NASBA HIV-1 QT) and two in-house assays (from National Genetics Institute and Baylor College of Medicine) were compared with a blinded panel. The results were evaluated as to intra-assay sensitivity, precision, and ability to detect differences in a dilution series.

* Corresponding author. Mailing address: Division of Molecular Virology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030. Phone: (713) 798-4454. Fax: (713) 798-3490. E-mail: blaineh{at}bcm.tmc.edu.

dagger Present address: Glaxo Wellcome, Inc., Research Triangle Park, NC 27709.

Journal of Clinical Microbiology, March 1998, p. 835-839, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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