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Journal of Clinical Microbiology, March 1998, p. 845-847, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of Two Enzyme-Linked Immunosorbent Assays and One Rapid Immunoblot Assay for Detection of Herpes Simplex Virus Type 2-Specific Antibodies in Serum

Jan Groen,1,* Grietje Van Dijk,2 Hubert G. M. Niesters,1 Willem I. Van Der Meijden,2 and Albert D. M. E. Osterhaus1

Institute of Virology1 and Department of Dermatology and Venerology,2 University Hospital Rotterdam, 3015 GD Rotterdam, The Netherlands

Received 16 July 1997/Returned for modification 19 August 1997/Accepted 24 November 1997

The sensitivities and specificities of three immunoassays for the detection of herpes simplex virus type 2 (HSV-2)-specific immunoglobulin G antibodies in serum, including the one-strip rapid immunoblot assay (RIBA; Chiron Corporation) and two indirect enzyme immunosorbent assays (EIA; Gull Laboratories and Centocor), were compared by testing a panel of 1,250 serum samples from individuals attending an outpatient clinic for sexually transmitted diseases. A qualitative agreement among the three assays was observed with 1,080 serum samples (86.4%); 291 of the serum samples (23.3%) were positive, 789 samples (63.1%) were negative, and 170 serum samples (13.6%) gave a discordant result. Results were considered conclusive when a concordant result was obtained with two of three assays. The sensitivities and specificities of the RIBA, the Gull EIA, and the Centocor EIA proved to be 99.2, 99.7, and 89.9% and 97.1, 96.7, and 99.3%, respectively. These results indicate that the Chiron RIBA and the Gull EIA are especially useful and reliable for the detection of HSV-2-specific antibodies in serum.

* Corresponding author. Mailing address: Institute of Virology, University Hospital Rotterdam, dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. Phone: 31.10.4635428. Fax: 31.10.4633441. E-mail: groen{at}viro.azr.nl.

Journal of Clinical Microbiology, March 1998, p. 845-847, Vol. 36, No. 3
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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