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Journal of Clinical Microbiology, April 1998, p. 1039-1041, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evaluation of PCR for Diagnosis of Melioidosis

Antje Haase,1,2,* Maree Brennan,2 Siobhan Barrett,2 Yvonne Wood,2 Sarah Huffam,1 Daniel O'Brien,1 and Bart Currie1,2

Royal Darwin Hospital, Tiwi, Northern Territory 0810,1 and Menzies School of Health Research, Casuarina, Northern Territory 0811,2 Australia

Received 2 July 1997/Returned for modification 17 November 1997/Accepted 6 January 1998

Previously published PCR-based diagnostic tests for melioidosis were evaluated for clinical usefulness. A Burkholderia pseudomallei 16S rRNA-derived primer set had a sensitivity approaching 100% for clinical samples from 22 culture-confirmed cases of melioidosis and enabled diagnosis of 3 culture-negative cases. However, samples from 10 of 30 inpatients from Royal Darwin Hospital with other diagnoses were positive by PCR, giving a specificity of 67% and a positive predictive value of only 70%. Although there are a number of intriguing possible explanations for our results, concerns of inappropriate therapy resulting from a positive result by PCR have led us to forgo the advantage of rapid PCR diagnosis for melioidosis until a better system is validated.

* Corresponding author. Mailing address: Royal Darwin Hospital, Pathology Labs, Biochemistry, Rocklands Dr., Tiwi, NT 0810, Australia. Phone: 61-8-89228085. Fax: 61-8-89228843. E-mail: antje.haase{at}nt.gov.au.

Journal of Clinical Microbiology, April 1998, p. 1039-1041, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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