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Journal of Clinical Microbiology, April 1998, p. 1081-1082, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Glycogen Assay for Diagnosis of Female Genital Chlamydia trachomatis Infection

Yang Chun* and Zhu Dao Yin

Department of Microbiology, Chongqing University of Medical Sciences, Chongqing 400042, People's Republic of China

Received 23 October 1997/Returned for modification 17 November 1997/Accepted 15 December 1997

Chlamydia trachomatis can synthesize glycogen at various stages in its developmental cycle. The glycogen content of female genital epithelial cells was detected by anthrone, and the results were compared with those from PCR. A total of 320 cervical samples were examined. Of 92 specimens that were positive by PCR, 78 were positive and 14 were negative by the glycogen assay. Of 228 specimens that were negative by PCR, 220 were negative and 8 were positive by the glycogen assay. The sensitivity and specificity of the glycogen assay obtained from these data were 84.8% (78 of 92) and 96.5% (220 of 228), respectively. Use of the glycogen assay to detect the glycogen content in genital epithelial cells may be helpful in the diagnosis of C. trachomatis infection. This is an easy, fast, and inexpensive assay and can be done in less-sophisticated labs.

* Corresponding author. Mailing address: Department of Microbiology, Chongqing University of Medical Sciences, Chongqing 400042, People's Republic of China. Phone: 86-23-68803788. Fax: 86-23-67858357. E-mail: wangyi{at}public.cta.cq.cn.

Journal of Clinical Microbiology, April 1998, p. 1081-1082, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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