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Journal of Clinical Microbiology, April 1998, p. 1122-1124, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Spoligotyping Followed by Double-Repetitive-Element PCR as Rapid Alternative to IS6110 Fingerprinting for Epidemiological Studies of Tuberculosis

Christophe Sola, Lionel Horgen, Jerome Maïsetti, Anne Devallois, Khye Seng Goh, and Nalin Rastogi*

Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Morne Jolivière, 97165 Pointe-à-Pitre, Guadeloupe, French West Indies

Received 31 October 1997/Returned for modification 22 December 1997/Accepted 14 January 1998

A total of 129 clinical isolates of Mycobacterium tuberculosis representing 91 patients were typed by a combination of direct-repeat (DR)-based spoligotyping and an inter-IS6110-PGRS (polymorphic GC-rich region)-PCR, also designated double-repetitive-element PCR (DRE-PCR). During the first phase of this investigation, 72 clinical strains representing 52 patients were initially typed by IS6110-restriction fragment length polymorphism (RFLP) and DR-RFLP, followed by spoligotyping and DRE-PCR. In the second phase of this investigation, the discriminating ability of spoligotyping plus DRE-PCR was studied for 57 isolates from 39 patients who were suspected to be epidemiologically linked, and the typing results were later confirmed by IS6110-RFLP and DR-RFLP analyses. The molecular clustering of the isolates remained identical irrespective of the methods used. These results show that the association of two PCR-based fingerprinting techniques for molecular epidemiology of tuberculosis has a discriminating ability similar to the IS6110-RFLP reference method.

* Corresponding author. Mailing address: Institut Pasteur, Morne Jolivière, B.P. 484, F-97165 Pointe-à-Pitre Cedex, Guadeloupe, French West Indies. Phone: 590-893-881. Fax: 590-893-880. E-mail: rastogi{at}ipagua.gp.

Journal of Clinical Microbiology, April 1998, p. 1122-1124, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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