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Journal of Clinical Microbiology, April 1998, p. 1139-1140, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Long-Term Survival of Streptococcus pneumoniae at Room Temperature on Dorset Egg Medium

Avril D. Wasas, Robin E. Huebner,* Mignon De Blanche, and Keith P. Klugman

MRC/SAIMR/WITS Pneumococcal Diseases Research Unit, South African Institute for Medical Research, Hillbrow, Johannesburg, South Africa

Received 15 September 1997/Returned for modification 11 November 1997/Accepted 23 December 1997

Forty-five isolates of Streptococcus pneumoniae were inoculated on Dorset egg and supplemented Columbia agar base media, incubated overnight at 37°C, and then kept at room temperature (RT; 21°C) or 4°C. Long-term viability was best at RT for both media, with all isolates remaining viable on Dorset egg medium for 44 days; viability was 90 and 57% on Columbia agar base medium after 7 and 30 days. We recommend the use of Dorset egg medium for the maintenance of pneumococci at RT.

* Corresponding author. Mailing address: South African Institute for Medical Research, P.O. Box 1038, Johannesburg 2000, South Africa. Phone: 27-11-489-9365. Fax: 27-11-489-9012. E-mail: 178hue{at}chiron.wits.ac.za.

Journal of Clinical Microbiology, April 1998, p. 1139-1140, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Charalambous, B. M., Batt, S. L., Peek, A. C., Mwerinde, H., Sam, N., Gillespie, S. H. (2003). Quantitative Validation of Media for Transportation and Storage of Streptococcus pneumoniae. J. Clin. Microbiol. 41: 5551-5556 [Abstract] [Full Text]  
  • Wasas, A. D., Huebner, R. E., Klugman, K. P. (1999). Use of Dorset Egg Medium for Maintenance and Transport of Neisseria meningitidis and Haemophilus influenzae Type b. J. Clin. Microbiol. 37: 2045-2046 [Abstract] [Full Text]  


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