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Journal of Clinical Microbiology, April 1998, p. 1157-1159, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Chromogenic Tube Test for Presumptive Identification or Confirmation of Isolates as Candida albicans

John Merlino,1,2,* Evanthia Tambosis,1 and Duncan Veal2

Department of Microbiology and Infectious Diseases, Concord Repatriation General Hospital, Concord, New South Wales 2139,1 and School of Biological Sciences, Macquarie University, New South Wales 2109,2 Australia

Received 21 July 1997/Returned for modification 9 September 1997/Accepted 22 December 1997

This report describes a new, modified, simple, and cost-effective method for the use of CHROMagar Candida (CHROMagar Company, Paris, France) for the presumptive identification of isolates as Candida albicans after preliminary growth. Sixty randomly selected clinical isolates were evaluated, including 38 of C. albicans. With incubation at 37°C for 24 h, the sensitivity and specificity appeared to be excellent and the test performed better than the traditional germ tube test. However, at earlier times, C. tropicalis isolates gave false-positive results.


* Corresponding author. Mailing address: Department of Microbiology and Infectious Diseases, Concord Repatriation General Hospital, Hospital Rd., Concord, NSW 2139, Australia. Phone: (612) 9767 6658. Fax: (612) 9767 7868. E-mail: john{at}micr.crg.cs.nsw.gov.au.


Journal of Clinical Microbiology, April 1998, p. 1157-1159, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.



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