This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gardiner, D. L.
Right arrow Articles by Sriprakash, K. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gardiner, D. L.
Right arrow Articles by Sriprakash, K. S.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 1998, p. 902-907, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Group A Streptococcal Vir Types Are M-Protein Gene (emm) Sequence Type Specific

Don L. Gardiner,1,* Alison M. Goodfellow,1 Diana R. Martin,2 and Kadaba S. Sriprakash1

Menzies School of Health Research, Darwin, Australia,1 and ESR, Communicable Disease Centre, Porirua, New Zealand2

Received 10 September 1997/Returned for modification 16 October 1997/Accepted 14 December 1997

The M-protein genes (emm genes) of 103 separate impetiginous Streptococcus pyogenes isolates were sequenced and the sequence types were compared to the types obtained by Vir typing. Vir typing is based on restriction fragment length polymorphism (RFLP) analysis of a 4- to 7-kb pathogenicity island encoding emm and other virulence genes. By using both HaeIII and HinfI to generate RFLP profiles, complete concordance between Vir type and emm sequence type was found. Comparison of the emm sequences with those in GenBank revealed new sequence types sharing less than 90% identity with known types. Diversity in the emm sequence was generated by corrected frameshift mutations, point mutations, and small in-frame mutations.

* Corresponding author. Mailing address: Menzies School of Health Research, Combined Health Building, Building 58, Royal Darwin Hospital, Rocklands Dr., Tiwi 0812, Northern Territory, Australia. Phone: 61-8-89228006. Fax: 61-8-89275187. E-mail don{at}menzies.su.edu.au.

Journal of Clinical Microbiology, April 1998, p. 902-907, Vol. 36, No. 4
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Luca-Harari, B., Straut, M., Cretoiu, S., Surdeanu, M., Ungureanu, V., van der Linden, M., Jasir, A. (2008). Molecular characterization of invasive and non-invasive Streptococcus pyogenes isolates from Romania. J Med Microbiol 57: 1354-1363 [Abstract] [Full Text]  
  • Lloyd, C., Balakrishnan, N., Menon, T. (2008). vir types of Streptococcus pyogenes in Chennai, South India. J Med Microbiol 57: 1176-1177 [Full Text]  
  • Sahand, I. H., Moragues, M. D., Eraso, E., Villar-Vidal, M., Quindos, G., Ponton, J. (2005). Supplementation of CHROMagar Candida Medium with Pal's Medium for Rapid Identification of Candida dubliniensis. J. Clin. Microbiol. 43: 5768-5770 [Abstract] [Full Text]  
  • Tewodros, W., Kronvall, G. (2005). M Protein Gene (emm Type) Analysis of Group A Beta-Hemolytic Streptococci from Ethiopia Reveals Unique Patterns. J. Clin. Microbiol. 43: 4369-4376 [Abstract] [Full Text]  
  • Teixeira, L. M., Barros, R. R., Castro, A. C. D., Peralta, J. M., Da Gloria S. Carvalho, M., Talkington, D. F., Vivoni, A. M., Facklam, R. R., Beall, B. (2001). Genetic and Phenotypic Features of Streptococcus pyogenes Strains Isolated in Brazil That Harbor New emm Sequences. J. Clin. Microbiol. 39: 3290-3295 [Abstract] [Full Text]  
  • Brandt, E. R., Teh, T., Relf, W. A., Hobb, R. I., Good, M. F. (2000). Protective and Nonprotective Epitopes from Amino Termini of M Proteins from Australian Aboriginal Isolates and Reference Strains of Group A Streptococci. Infect. Immun. 68: 6587-6594 [Abstract] [Full Text]  
  • Kataja, J., Huovinen, P., The Macrolide Resistance Study Group, , Seppala, H. (2000). Erythromycin resistance genes in group A streptococci of different geographical origins. J Antimicrob Chemother 46: 789-792 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»