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Journal of Clinical Microbiology, May 1998, p. 1176-1179, Vol. 36, No. 5
University of Texas Medical Branch,
Galveston, Texas
Received 8 October 1997/Returned for modification 6 December
1997/Accepted 2 February 1998
The reliability of MYCO/F Lytic medium in the BACTEC 9240 blood
culture system was evaluated by comparing its performance to that of
the Isolator system for the recovery of fungi and to that of the ESP II
system for the recovery of mycobacteria. Of 717 specimens of blood
cultured for fungi, 24 were positive; 12 samples were positive with
both systems, 7 samples were positive with the Isolator system only,
and 5 samples were positive with MYCO/F Lytic medium only. Fourteen
samples grew Histoplasma capsulatum; both systems detected
H. capsulatum in seven samples but the Isolator system
alone detected H. capsulatum in seven samples. The mean times to the detection of H. capsulatum were 8 days (range,
4 to 13 days) for MYCO/F Lytic medium and 9 days (range, 6 to 18 days)
for the Isolator system; the mean times to identification were 20 days
(range, 15 to 24 days) for isolates recovered with MYCO/F Lytic medium
and 11 days (range, 6 to 18 days) for those recovered with the Isolator
system (P < 0.05). Cryptococcus
neoformans was isolated from 10 fungal cultures; five isolates
grew in both systems, and five isolates grew in MYCO/F Lytic medium
only. The mean times to detection of C. neoformans were 4 days (range, 2 to 6 days) for MYCO/F Lytic medium and 7 days (range, 5 to 7 days) for the Isolator system (P < 0.05); the
mean times to identification were 15 days (range, 7 to 27 days) for
isolates recovered with MYCO/F Lytic medium and 8 days (range, 7 to 11 days) for those recovered with the Isolator system. Of the 687 samples
of blood cultured for mycobacteria, 64 blood samples from 42 patients
grew mycobacteria (58 grew Mycobacterium avium complex, 4 grew Mycobacterium kansasii, and 2 grew Mycobacterium
tuberculosis); 42 isolates were recovered with both systems, 18 were isolated with MYCO/F medium only, and 4 were isolated with the ESP
II system only alone (P < 0.05). The mean time to
detection of mycobacteria with MYCO/F Lytic medium was 14 days, whereas
it was 17 days with the ESP II system (P < 0.05). In
summary, the combination of MYCO/F Lytic medium and the BACTEC 9240 instrument is an excellent blood culture system for the growth and
detection of mycobacteria. A valid assessment of MYCO/F Lytic medium
with regard to fungal isolation, however, was not possible due to the
small number of isolates recovered.
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Evaluation of BACTEC MYCO/F Lytic Medium for
Recovery of Mycobacteria and Fungi from Blood
and
*
Corresponding author. Mailing address: Department of
Pathology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0740. Phone: (409) 772-4851. Fax: (409) 772-5683. E-mail: gwoods{at}utmb.edu.
Present address: Promega Corp., Madison, WI 53711-5399.
Journal of Clinical Microbiology, May 1998, p. 1176-1179, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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