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Journal of Clinical Microbiology, May 1998, p. 1189-1192, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Immunoglobulin A-Specific Capture Enzyme-Linked Immunosorbent Assay for Diagnosis of Dengue Fever

Antoine Talarmin,* Bhety Labeau, Josiane Lelarge, and Jean-Louis Sarthou

Centre National de Référence pour la Surveillance de la Dengue et de la Fièvre Jaune, Institut Pasteur de la Guyane, Cayenne, French Guiana

Received 4 November 1997/Returned for modification 16 December 1997/Accepted 26 January 1998

Dengue fever (DF) is usually diagnosed by testing for dengue virus immunoglobulin M (IgM) by a capture enzyme-linked immunosorbent assay (ELISA) (MAC-ELISA). However, IgM can last for months, and its presence might reflect a previous infection. We have tested the use of anti-dengue virus IgA capture ELISA (AAC-ELISA) for the diagnosis of DF by comparing the results of MAC-ELISAs and AAC-ELISAs for 178 serum samples taken from patients with confirmed cases of DF. IgM appears more rapidly (mean delay of positivity, 3.8 days after the onset of DF) than IgA (4.6 days) but lasts longer; the peak IgA titer is obtained on day 8. The specificity and the positive predictive value of AAC-ELISA are 100%; its sensitivity and negative predictive value (NPV) are also 100% between days 6 and 25 after the onset of DF, but they decrease drastically when data for tests conducted with specimens from the first days of infection are included, because the IgA titers, like the IgM titers, have not yet risen. AAC-ELISA is a simple method that can be performed together with MAC-ELISA and that can help in interprating DF serology.

* Corresponding author. Mailing address: Institut Pasteur de la Guyane, BP 6010, 97306 Cayenne cedex, French Guiana. Phone: (594) 29.26.09. Fax: (594) 30.94.16.

Journal of Clinical Microbiology, May 1998, p. 1189-1192, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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