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Journal of Clinical Microbiology, May 1998, p. 1404-1407, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Identification of Acinetobacters on Blood Agar in Presence of D-Glucose by Unique Browning Effect

Hong Siau,* Kwok-Yung Yuen, Pak-Leung Ho, Wei-Kwang Luk, Samson S. Y. Wong, Patrick C. Y. Woo, Rodney A. Lee, and Wai-Ting Hui

Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Hong Kong, China

Received 14 October 1997/Returned for modification 9 January 1998/Accepted 6 February 1998

A positive phenotypic characteristic of glucose-oxidizing acinetobacters was demonstrated with blood agar containing D-glucose. Glucose-oxidizing Acinetobacter baumannii, Acinetobacter genospecies 3, Acinetobacter lwoffii, and Acinetobacter genospecies 13 sensu Tjernberg and Ursing caused a unique brown discoloration of media supplemented with 5% blood (of horse, sheep, or human origin) and an aldose sugar (0.22 M D-glucose, D-galactose, D-mannose, D-xylose, or lactose). The browning effect was not observed when a ketose sugar (D-fructose or sucrose) was substituted for the aldose sugar or under high osmolarity in the presence of mannitol, glycerol, or sodium chloride. Other gram-negative nonfermenters (non-glucose-oxidizing acinetobacters, Pseudomonas aeruginosa, other Pseudomonas spp., Stenotrophomonas maltophilia, Flavobacterium spp., and Moraxella spp.) did not cause similar discoloration. This novel browning effect may serve as an alternative trait for identifying glucose-oxidizing acinetobacters.

* Corresponding author. Mailing address: Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital Compound, Pokfulam Rd., Hong Kong, China. Phone: (852) 2855 4823. Fax: (852) 2855 1241. E-mail: hongsiau{at}hkusua.hku.hk.

Journal of Clinical Microbiology, May 1998, p. 1404-1407, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.




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