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Journal of Clinical Microbiology, May 1998, p. 1425-1427, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Buffered Plate Antigen Test as a Screening Test for Diagnosis of Human Brucellosis

Nidia E. Lucero1,* and Jorge E. Bolpe2

Instituto Nacional de Microbiología "Dr. C. G. Malbrán,"1 and Departamento de Zoonosis Rurales, España 770, 7300 Azul,2 Buenos Aires, Argentina

Received 27 May 1997/Returned for modification 12 July 1997/Accepted 31 January 1998

Brucellosis in Argentina is currently investigated in bank donor blood by the standard plate agglutination test (PAT). This study evaluated the buffered plate antigen test (BPA), now used to screen for bovine brucellosis, as a screen for human disease. Of 57 sera from patients with culture-confirmed brucellosis, 100% were detected with the BPA. Of 142 sera positive by rose bengal (RB) and complement fixation (CF), from patients with clinical evidence of brucellosis, the BPA detected 100%. Of 307 sera from a nonsymptomatic population that were RB and CF negative, the BPA detected 99.67% of the negative sera. The data indicate that the BPA is satisfactory compared to the other agglutination tests employed. It is an inexpensive and practical screening test and reduces the nonspecific reactions detected by the PAT.

* Corresponding author. Mailing address: Instituto Nacional de Microbiología "Dr. C. G. Malbrán," Velez Sarsfield 563, 1281 Buenos Aires, Argentina. Phone: 54-1-303-1806/11. Fax: 54-1-303-2382. E-mail: NIDIA{at}IMPSAT1.COM.AR.

Journal of Clinical Microbiology, May 1998, p. 1425-1427, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jacob, N. R., Rodriguez, C. G., Binaghi, M. A., Scapellato, P. G., Rosales Ostriz, M. B., Ayala, S. M., Lucero, N. E. (2008). Brucellosis complicating chronic non-infectious disorders: diagnostic and therapeutic dilemmas. J Med Microbiol 57: 1161-1166 [Abstract] [Full Text]  
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  • Lucero, N. E., Foglia, L., Ayala, S. M., Gall, D., Nielsen, K. (1999). Competitive Enzyme Immunoassay for Diagnosis of Human Brucellosis. J. Clin. Microbiol. 37: 3245-3248 [Abstract] [Full Text]  


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