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Journal of Clinical Microbiology, May 1998, p. 1443-1445, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Comparison of the Rapid Yeast Plus Panel with the API20C Yeast System for Identification of Clinically Significant Isolates of Candida Species

Judith S. Heelan,1,2,* Edgar Sotomayor,1 Kimberly Coon,1 and Julia B. D'Arezzo3

Department of Pathology, Memorial Hospital of Rhode Island, Pawtucket, Rhode Island 028601; Department of Pathology, Veterans' Administration Medical Center, Providence, Rhode Island 029083; and Department of Pathology and Laboratory Medicine, Brown University School of Medicine, Providence, Rhode Island 029012

Received 30 December 1997/Returned for modification 26 January 1998/Accepted 3 February 1998

The RapID Yeast Plus system (Innovative Diagnostic Systems, Norcross, Ga.) is a qualitative micromethod employing conventional tests and single-substrate chromogenic tests and having a 4-h incubation period. This system was compared with the API20C (bioMerieux Vitek, Hazelwood, Mo.) system, a 24- to 72-h carbohydrate assimilation method. One hundred thirty-three clinical yeast isolates, including 57 of Candida albicans, 26 of Candida tropicalis, 23 of Candida glabrata, and 27 of other yeasts, were tested by both methods. When discrepancies occurred, isolates were further tested by the Automated Yeast Biochemical Card (bioMerieux Vitek). Germ tube production and microscopic morphology were used as needed to definitively identify yeast isolates. The RapID Yeast Plus system correctly identified 125 yeast isolates, with an overall accuracy of 94% (125 of 133). Excellent correlation was found in the recognition of the three yeasts most commonly isolated from human sources. The test was 99% (105 of 106 isolates) accurate with C. albicans, C. tropicalis, and C. glabrata. The RapID Yeast Plus system compares favorably with the API20C system and provides a simple, accurate alternative to conventional assimilation methods for the rapid identification of the most commonly encountered isolates of Candida species.

* Corresponding author. Mailing address: Microbiology Laboratory, Memorial Hospital of Rhode Island, 111 Brewster St., Pawtucket, RI 02860. Phone: (401) 729-2839. Fax: (401) 729-2990.

Journal of Clinical Microbiology, May 1998, p. 1443-1445, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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