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Journal of Clinical Microbiology, May 1998, p. 1446-1449, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Survey of Klebsiella pneumoniae Strains Producing Extended-Spectrum beta -Lactamases: Prevalence of SHV-12 and SHV-2a in Korea

Jungmin Kim,1,* Youngmi Kwon,1 Hyunjoo Pai,2 Jong-Won Kim,3 and Dong-Taek Cho4

Department of Microbiology1 and Department of Internal Medicine,2 College of Medicine, Dankook University, Chonan, Department of Clinical Pathology, College of Medicine, Sungkyunkwan University, Seoul,3 and Department of Microbiology, College of Medicine, Kyungpook University, Taegu,4 Korea

Received 23 October 1997/Returned for modification 20 January 1998/Accepted 20 February 1998

Fifty-three clinical isolates of extended-spectrum beta -lactamase (ESBL)-producing Klebsiella pneumoniae collected from three hospitals in Korea were investigated for phenotypical and genotypical characterizations. Among these, 39 strains (74%) were shown by isoelectric focusing to carry SHV-type beta -lactamases: 27 strains showed the pI 8.2 beta -lactamase, and another 12 strains showed the pI 7.6 beta -lactamase. The SHV gene of each of these strains was amplified by PCR, followed by nucleotide sequencing analysis. The gene of the pI 8.2 beta -lactamase was found to be identical to the sequences encoding SHV-12, and the gene of the pI 7.6 beta -lactamase was identical to the sequences encoding SHV-2a. A total of eight cefoxitin-resistant strains were found to have the plasmid-mediated AmpC-type beta -lactamase, with a pI of 8.0, and this was confirmed to be CMY-1 beta -lactamase by PCR and hybridization analysis. Noteworthy in this study is the fact that SHV-12 and SHV-2a have been the most commonly identified SHV-type ESBLs in Korea.

* Corresponding author. Mailing address: Department of Microbiology, College of Medicine, Dankook University, San 29, Anseo-dong, Chonan, Chungnam, 330-180, Korea. Phone: 82-417-550-3853. Fax: 82-417-550-3905. E-mail: minkim{at}ns.anseo.dankook.ac.kr.

Journal of Clinical Microbiology, May 1998, p. 1446-1449, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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