This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sugita, T.
Right arrow Articles by Shinoda, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sugita, T.
Right arrow Articles by Shinoda, T.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 1998, p. 1458-1460, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Rapid Detection of Species of the Opportunistic Yeast Trichosporon by PCR

Takashi Sugita, Akemi Nishikawa, and Takako Shinoda*

Department of Microbiology, Meiji College of Pharmacy, Tanashi, Tokyo, Japan

Received 4 August 1997/Returned for modification 7 October 1997/Accepted 30 January 1998

Trichosporon species are opportunistic pathogens, associated with a high mortality rate in immunocompromised patients. Oligonucleotide primers were used to amplify a 170-bp fragment of small-subunit ribosomal DNA of all species in the genus Trichosporon by PCR. The primers amplify DNAs of all species in the genus Trichosporon, including six causative agents of trichosporonosis. DNAs of other medically important yeasts, such as Candida albicans and Cryptococcus neoformans, are not amplified by this detection system.

* Corresponding author. Mailing address: Department of Microbiology, Meiji College of Pharmacy, 1-22-1 Yato-cho, Tanashi, Tokyo 188-0001 Japan. Phone: 81-424-21-0339. Fax: 81-424-21-1489. E-mail: shinoda{at}my-pharm.ac.jp.

Journal of Clinical Microbiology, May 1998, p. 1458-1460, Vol. 36, No. 5
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chagas-Neto, T. C., Chaves, G. M., Melo, A. S. A., Colombo, A. L. (2009). Bloodstream Infections Due to Trichosporon spp.: Species Distribution, Trichosporon asahii Genotypes Determined on the Basis of Ribosomal DNA Intergenic Spacer 1 Sequencing, and Antifungal Susceptibility Testing. J. Clin. Microbiol. 47: 1074-1081 [Abstract] [Full Text]  
  • Kardjeva, V., Summerbell, R., Kantardjiev, T., Devliotou-Panagiotidou, D., Sotiriou, E., Graser, Y. (2006). Forty-Eight-Hour Diagnosis of Onychomycosis with Subtyping of Trichophyton rubrum Strains. J. Clin. Microbiol. 44: 1419-1427 [Abstract] [Full Text]  
  • Ahmad, S., Al-Mahmeed, M., Khan, Z. U (2005). Characterization of Trichosporon species isolated from clinical specimens in Kuwait. J Med Microbiol 54: 639-646 [Abstract] [Full Text]  
  • Diaz, M. R., Fell, J. W. (2004). High-Throughput Detection of Pathogenic Yeasts of the Genus Trichosporon. J. Clin. Microbiol. 42: 3696-3706 [Abstract] [Full Text]  
  • Abliz, P., Fukushima, K., Takizawa, K., Nieda, N., Miyaji, M., Nishimura, K. (2003). Rapid Identification of the Genus Fonsecaea by PCR with Specific Oligonucleotide Primers. J. Clin. Microbiol. 41: 873-876 [Abstract] [Full Text]  
  • Sugita, T., Nishikawa, A., Ikeda, R., Shinoda, T. (1999). Identification of Medically Relevant Trichosporon Species Based on Sequences of Internal Transcribed Spacer Regions and Construction of a Database for Trichosporon Identification. J. Clin. Microbiol. 37: 1985-1993 [Abstract] [Full Text]  
  • Sugita, T., Nishikawa, A., Shinoda, T. (1998). Identification of Trichosporon asahii by PCR Based on Sequences of the Internal Transcribed Spacer Regions. J. Clin. Microbiol. 36: 2742-2744 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»