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Journal of Clinical Microbiology, June 1998, p. 1574-1577, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Mapping and Serodiagnostic Application of a Dominant Epitope within the Human Herpesvirus 8 ORF 65-Encoded Protein

Chou-Pong Pau,1,* Lee L. Lam,1 Thomas J. Spira,1 Jodi B. Black,2 John A. Stewart,2 Philip E. Pellett,2 and Richard A. Respess1

Division of AIDS, STD, and TB Laboratory Research1 and Division of Viral and Rickettsial Diseases,2 National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333

Received 22 December 1997/Returned for modification 11 February 1998/Accepted 11 March 1998

A dominant epitope within the human herpesvirus 8 (HHV8) ORF 65-encoded protein was mapped to an 8-amino-acid (aa) sequence (RKPPSGKK [aa 162 to 169]) by an amino acid replacement method. Using a 14-aa peptide (P4) encompassing this epitope as the antigen, we developed an enzyme immunoassay for HHV8 antibodies. The presence of P4 antibodies in a panel of 61 human serum specimens was highly correlated with biopsy-confirmed Kaposi's sarcoma. The homologous Epstein-Barr virus peptide derived from BFBR3-encoded protein did not interfere with the assay, suggesting that P4 is specific for HHV8.

* Corresponding author. Mailing address: Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, 1600 Clifton Rd., Mail Stop D12, Atlanta, GA 30333. Phone: (404) 639-3765. Fax: (404) 639-2660. E-mail: CHP3{at}CDC.GOV.

Journal of Clinical Microbiology, June 1998, p. 1574-1577, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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