This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Verweij, P. E.
Right arrow Articles by Meis, J. F. G. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Verweij, P. E.
Right arrow Articles by Meis, J. F. G. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 1998, p. 1612-1616, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Detection of Antigen in Sera of Patients with Invasive Aspergillosis: Intra- and Interlaboratory Reproducibility

Paul E. Verweij,1,* Zoran Erjavec,2 Wim Sluiters,3 Wil Goessens,4 Marja Rozenberg-Arska,5 Yvette J. Debets-Ossenkopp,6 Henri F. L. Guiot,7 and Jacques F. G. M. Meis1 for The Dutch Interuniversity Working Party for Invasive Mycoses

Department of Medical Microbiology, University Hospital Nijmegen, Nijmegen,1 Departments of Hematology2 and Medical Statistics,3 University Hospital Groningen, Groningen, Department of Medical Microbiology and Infectious Diseases, University Hospital Rotterdam, Rotterdam,4 Department of Medical Microbiology, University Hospital Utrecht, Utrecht,5 Department of Clinical Microbiology and Infection Control, Free University Hospital, Amsterdam,6 and Department of Infectious Diseases, University Hospital Leiden, Leiden,7 The Netherlands

Received 16 December 1997/Returned for modification 9 February 1998/Accepted 10 March 1998

The intra- and interlaboratory reproducibilities of a commercial sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of Aspergillus galactomannan in serum (Platelia Aspergillus; Sanofi Diagnostics Pasteur, Marnes-La-Coquette, France) were evaluated in six laboratories of university hospitals. Twenty serum samples were obtained from 12 neutropenic patients including 6 with invasive aspergillosis. These samples were blinded and sent to each center together with eight blinded ELISA-negative serum samples spiked with known concentrations of galactomannan. The centers were provided with ELISA microtiter plates from a single batch and a detailed protocol. Ten clinical samples showed ELISA reactivity, while 10 samples were ELISA negative. The mean coefficient of variation (CV) of the optical density values was 4.24% within a single assay and 25.6% between runs. The interassay CV of the ratios for the serum samples tested was 18.6%. Analysis of ordinal interpretation of the ELISA result (i.e., negative, gray zone, or positive) showed excellent reproducibility. Recalculation of the cutoff values for positive and negative samples suggested that the cutoff level recommended by the manufacturer could be lowered from 1.0 to 0.8 for negative samples and from 1.5 to 1.0 for positive samples. The intra- and interlaboratory reproducibilities were excellent when the ELISA results were interpreted as ordinal data, but considerable variation in optical density values and, to a lesser extent, in the ratios for the serum samples tested, was observed between runs. High assay variability was also found for serum samples spiked with known concentrations of galactomannan. Therefore, antigen titers in serum samples from a single patient, measured in different runs, should be compared with caution.

* Corresponding author. Mailing address: Department of Medical Microbiology, University Hospital Nijmegen, P.O. Box 9101, Geert Grooteplein 24, 6500 HB Nijmegen, The Netherlands. Phone: 31-24-3614356. Fax: 31-24-3540216. E-mail: p.verweij{at}mmb.azn.nl.

Journal of Clinical Microbiology, June 1998, p. 1612-1616, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Bart-Delabesse, E., Basile, M., Al Jijakli, A., Souville, D., Gay, F., Philippe, B., Bossi, P., Danis, M., Vernant, J.-P., Datry, A. (2005). Detection of Aspergillus Galactomannan Antigenemia To Determine Biological and Clinical Implications of Beta-Lactam Treatments. J. Clin. Microbiol. 43: 5214-5220 [Abstract] [Full Text]  
  • Upton, A., Gugel, A., Leisenring, W., Limaye, A., Alexander, B., Hayden, R., Marr, K. A. (2005). Reproducibility of Low Galactomannan Enzyme Immunoassay Index Values Tested in Multiple Laboratories. J. Clin. Microbiol. 43: 4796-4800 [Abstract] [Full Text]  
  • Walsh, T. J., Shoham, S., Petraitiene, R., Sein, T., Schaufele, R., Kelaher, A., Murray, H., Mya-San, C., Bacher, J., Petraitis, V. (2004). Detection of Galactomannan Antigenemia in Patients Receiving Piperacillin-Tazobactam and Correlations between In Vitro, In Vivo, and Clinical Properties of the Drug-Antigen Interaction. J. Clin. Microbiol. 42: 4744-4748 [Abstract] [Full Text]  
  • Singh, N., Obman, A., Husain, S., Aspinall, S., Mietzner, S., Stout, J. E. (2004). Reactivity of Platelia Aspergillus Galactomannan Antigen with Piperacillin-Tazobactam: Clinical Implications Based on Achievable Concentrations in Serum. Antimicrob. Agents Chemother. 48: 1989-1992 [Abstract] [Full Text]  
  • O'Sullivan, C. E., Kasai, M., Francesconi, A., Petraitis, V., Petraitiene, R., Kelaher, A. M., Sarafandi, A. A., Walsh, T. J. (2003). Development and Validation of a Quantitative Real-Time PCR Assay Using Fluorescence Resonance Energy Transfer Technology for Detection of Aspergillus fumigatus in Experimental Invasive Pulmonary Aspergillosis. J. Clin. Microbiol. 41: 5676-5682 [Abstract] [Full Text]  
  • Pinel, C., Fricker-Hidalgo, H., Lebeau, B., Garban, F., Hamidfar, R., Ambroise-Thomas, P., Grillot, R. (2003). Detection of Circulating Aspergillus fumigatus Galactomannan: Value and Limits of the Platelia Test for Diagnosing Invasive Aspergillosis. J. Clin. Microbiol. 41: 2184-2186 [Abstract] [Full Text]  
  • Latge, J.-P. (1999). Aspergillus fumigatus and Aspergillosis. Clin. Microbiol. Rev. 12: 310-350 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»