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Journal of Clinical Microbiology, June 1998, p. 1761-1764, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

16S rRNA Sequence Diversity in Mycobacterium celatum Strains Caused by Presence of Two Different Copies of 16S rRNA Gene

U. Reischl,1 K. Feldmann,2 L. Naumann,1 B. J. M. Gaugler,2 B. Ninet,3 B. Hirschel,3 and S. Emler3,*

Institute for Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg,1 Institute for Laboratory Diagnosis, Zentralkrankenhaus Gauting, D-82131 Gauting,2 Germany, and Laboratory of Bacteriology, Division of Infectious Diseases, Hopital Cantonal Universitaire, 1211 Geneva, Switzerland3

Received 29 August 1997/Returned for modification 6 October 1997/Accepted 13 February 1998

Direct sequencing of the 16S rRNA gene (16S rDNA) of Mycobacterium celatum isolates showed ambiguities, suggesting heterogeneity. Cloned 16S rDNA yielded two copies of the gene, which differed by insertion of a thymine at position 214 and by additional mismatches. Restriction fragment length polymorphism analysis confirmed the presence of two copies of 16S rDNA within the bacterial chromosome.

* Corresponding author. Mailing address: Laboratory of Bacteriology, Division of Infectious Diseases, University Hospital Geneva, CH-1211 Geneva 14, Switzerland. Phone: 41-22-37 23311 Bip 857 960. Fax: 41-22-37 27304. E-mail: Stefan.Emler{at}hcuge.ch.

Journal of Clinical Microbiology, June 1998, p. 1761-1764, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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