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Journal of Clinical Microbiology, June 1998, p. 1795-1797, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Multiplex PCR for Enterotoxigenic, Attaching and Effacing, and Shiga Toxin-Producing Escherichia coli Strains from Calves

Sophia M. Franck,1 Brad T. Bosworth,2 and Harley W. Moon1,*

Department of Pathology, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50011,1 and Enteric Diseases and Food Safety Research Unit, National Animal Disease Center, USDA Agricultural Research Service, Ames, Iowa 500102

Received 16 December 1997/Returned for modification 13 February 1998/Accepted 4 March 1998

A multiplex PCR was developed to identify enterotoxigenic, attaching and effacing, and Shiga toxin-producing Escherichia coli strains by amplifying genes encoding K99 and F41 fimbriae, heat-stable enterotoxin a, intimin, and Shiga toxins 1 and 2. This multiplex PCR was specific and sensitive. It will be useful for identification of E. coli strains which cause diarrhea in calves.

* Corresponding author. Mailing address: Veterinary Medical Research Inst., 1802 Elwood Dr., College of Veterinary Medicine, Ames, IA 50011-1240. Phone: (515) 294-9327. Fax: (515) 294-1401. E-mail: hmoon{at}iastate.edu.

Journal of Clinical Microbiology, June 1998, p. 1795-1797, Vol. 36, No. 6
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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