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Journal of Clinical Microbiology, July 1998, p. 1859-1863, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Characterization of IS1245 for Strain Typing of Mycobacterium avium

Martine Pestel-Caron1,2 and Robert D. Arbeit2,*

Groupe de Recherche sur les Anti-microbiens et les Micro-organismes, Laboratoire de Bactériologie, Centre Hospitalier Universitaire Charles Nicolle, F-76031 Rouen Cedex, France,1 and Research Service, Veterans Affairs Medical Center, Boston, Massachusetts 021302

Received 5 January 1998/Returned for modification 20 February 1998/Accepted 31 March 1998

IS1245 is an insertion element widely prevalent among isolates of Mycobacterium avium. We used PvuII Southern blots to analyze IS1245 polymorphisms among 159 M. avium isolates (141 clinical isolates from 40 human immunodeficiency virus-infected patients plus 18 epidemiologically related environmental isolates) that represented 40 distinct M. avium strains, as resolved by previous studies by pulsed-field gel electrophoresis (PFGE). All 40 strains carried DNA homologous to IS1245 and thus were typeable. Twenty-five (63%) strains had >= 10 copies of the element, 6 (15%) had 4 to 9 copies, and 9 (23%) had only 1 to 3 copies. Among the last group of nine strains (each of which was distinct by PFGE analysis), IS1245 typing resolved only four patterns and thus provided poor discriminatory power. To evaluate the in vivo stability of IS1245, we analyzed 32 strains for which sets of 2 to 19 epidemiologically related isolates were available. For 19 (59%) of these sets, all isolates representing the same strain had indistinguishable IS1245 patterns. Within eight (25%) sets, one or more isolates had IS1245 patterns that differed by one or two fragments from the modal pattern for the isolates of that strain. Five (16%) sets included isolates whose patterns differed by three or more fragments; on the basis of IS1245 typing those isolates would have been designated distinct strains. IS1245 was stable during in vitro passage, suggesting that the variations observed represented natural translocations of the element. IS1245 provides a useful tool for molecular strain typing of M. avium but may have limitations for analyzing strains with low copy numbers or for resolving extended epidemiologic relationships.

* Corresponding author. Mailing address: Research Service (151), VA Medical Center, 150 S. Huntington Ave., Boston, MA 02130. Phone: (617) 278-4416. Fax: (617) 739-6394. E-mail: rarbeit{at}bu.edu.

Journal of Clinical Microbiology, July 1998, p. 1859-1863, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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