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Journal of Clinical Microbiology, July 1998, p. 1907-1911, Vol. 36, No. 7
Laboratory of Bacteriology and Mycology,
Department of Pathology, Bacteriology and Avian Diseases, Faculty
of Veterinary Medicine, University of Ghent, B-9820 Merelbeke, Belgium
Received 23 September 1997/Returned for modification 31 December
1997/Accepted 7 April 1998
The influence of the addition of sheep blood to Mueller-Hinton II
agar and the effects of aerobic incubation with or without CO2 and of anaerobic incubation were tested with
bacitracin, tylosin, avoparcin, virginiamycin, avilamycin, narasin, and
flavomycin on enterococci. The antibacterial activity of bambermycin
(Flavomycin) was strongly inhibited by the addition of blood, except
with the species Enterococcus faecium, Enterococcus
mundtii, Enterococcus hirae, Enterococcus
casseliflavus, and Enterococcus gallinarum, which
were not susceptible to this antibiotic on blood-free medium. With all
other antimicrobials except avoparcin and tylosin, the presence of
blood resulted in MIC increases of 1 to 3 log2 differences. Incubation in aerobic or anaerobic atmospheres enriched with
CO2 lowered the susceptibility of enterococci to tylosin
and increased their susceptibility to avilamycin, narasin, and
avoparcin. This effect was most pronounced in tests on blood-free
media. Results of susceptibility tests incubated under anaerobiosis and
in a CO2-enriched atmosphere did not differ. For all
enterococcal species, the preferred conditions for testing the
susceptibility are Mueller-Hinton II medium supplemented with blood and
incubation in a CO2-enriched atmosphere. However, when only
E. faecium and Enterococcus faecalis are
being tested, Mueller-Hinton II medium without blood incubated aerobically gives satisfactory results.
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
Effects of Different Test Conditions on MICs of
Food Animal Growth-Promoting Antibacterial Agents for
Enterococci
*
Corresponding author. Mailing address: University of
Ghent, Faculty of Veterinary Medicine, Department of Pathology,
Bacteriology and Avian Diseases, Laboratory of Bacteriology and
Mycology, Salisburylaan 133, B-9820 Merelbeke, Belgium. Phone: 32 9 264 74 35. Fax: 32 9 264 74 94. E-mail:
pbutaye{at}allserv.rug.ac.be.
Journal of Clinical Microbiology, July 1998, p. 1907-1911, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.
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