This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Funke, G.
Right arrow Articles by Freney, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Funke, G.
Right arrow Articles by Freney, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 1998, p. 1948-1952, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Evaluation of the VITEK 2 System for Rapid Identification of Medically Relevant Gram-Negative Rods

Guido Funke,1,* Dominique Monnet,2,dagger Chiara deBernardis,1 Alexander von Graevenitz,1 and Jean Freney2

Department of Medical Microbiology, University of Zürich, CH-8028 Zürich, Switzerland,1 and UPRES EA 1655, Institut de Sciences Pharmaceutiques et Biologiques de Lyon, Faculté de Pharmacie Rockefeller, F-69373 Lyon Cedex 08, France2

Received 17 February 1998/Returned for modification 23 March 1998/Accepted 7 April 1998

The new VITEK 2 system (bioMérieux) was evaluated at two independent sites with the identification card for gram-negative bacilli (ID-GNB card). Of the 845 strains tested, which represented 70 different taxa belonging to either the family Enterobacteriaceae or the nonenteric bacilli, 716 (84.7%) were correctly identified at the species level. Thirty-two (3.8%) additional strains were identified to the species level after the performance of simple, rapid manual tests (oxidase, hemolysis, indole reaction, motility, and pigmentation). For 80 (9.5%) strains, these additional tests did not lead to an identification at the species level but the correct species identification was given among the organisms listed. Only 7 (0.8%) strains were misidentified, and 10 (1.2%) were not identified. Mistakes were randomly distributed over different taxa. Due to the new, more sensitive fluorescence-based technology of the VITEK 2 system, final results were available after 3 h. Since our evaluation was mainly a stress test, it is predicted that the VITEK 2 system in conjunction with the ID-GNB card would perform well under conditions of a routine clinical laboratory in identifying members of the family Enterobacteriaceae and selected species of nonenteric bacteria. This system is a promising, highly automated new tool for the rapid identification of gram-negative bacilli from human clinical specimens.

* Corresponding author. Mailing address: Department of Medical Microbiology, University of Zürich, Gloriastrasse 32, CH-8028 Zürich, Switzerland. Phone: +41-1-634-2701. Fax: +41-1-634-4906. E-mail: funke{at}immv.unizh.ch.

dagger Present address: Department of Gastrointestinal Infections, Statens Serum Institut, 2300 Copenhagen S, Denmark.

Journal of Clinical Microbiology, July 1998, p. 1948-1952, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Otto-Karg, I., Jandl, S., Muller, T., Stirzel, B., Frosch, M., Hebestreit, H., Abele-Horn, M. (2009). Validation of Vitek 2 Nonfermenting Gram-Negative Cards and Vitek 2 Version 4.02 Software for Identification and Antimicrobial Susceptibility Testing of Nonfermenting Gram-Negative Rods from Patients with Cystic Fibrosis. J. Clin. Microbiol. 47: 3283-3288 [Abstract] [Full Text]  
  • Zbinden, A., Bottger, E. C., Bosshard, P. P., Zbinden, R. (2007). Evaluation of the Colorimetric VITEK 2 Card for Identification of Gram-Negative Nonfermentative Rods: Comparison to 16S rRNA Gene Sequencing. J. Clin. Microbiol. 45: 2270-2273 [Abstract] [Full Text]  
  • Bosshard, P. P., Zbinden, R., Abels, S., Boddinghaus, B., Altwegg, M., Bottger, E. C. (2006). 16S rRNA Gene Sequencing versus the API 20 NE System and the VITEK 2 ID-GNB Card for Identification of Nonfermenting Gram-Negative Bacteria in the Clinical Laboratory. J. Clin. Microbiol. 44: 1359-1366 [Abstract] [Full Text]  
  • Janda, J. M., Abbott, S. L. (2006). The Genus Hafnia: from Soup to Nuts. Clin. Microbiol. Rev. 19: 12-28 [Abstract] [Full Text]  
  • Wallet, F., Loiez, C., Renaux, E., Lemaitre, N., Courcol, R. J. (2005). Performances of VITEK 2 Colorimetric Cards for Identification of Gram-Positive and Gram-Negative Bacteria. J. Clin. Microbiol. 43: 4402-4406 [Abstract] [Full Text]  
  • Bruins, M. J., Wolfhagen, M. J. H. M. (2005). Nonfermenters under the Influence: Ethanol Disinfection of a Saline Dispenser Caused Misidentification by the Vitek 2 System Using the New GN Cards. J. Clin. Microbiol. 43: 4296-4297 [Full Text]  
  • O'Hara, C. M. (2005). Manual and Automated Instrumentation for Identification of Enterobacteriaceae and Other Aerobic Gram-Negative Bacilli. Clin. Microbiol. Rev. 18: 147-162 [Abstract] [Full Text]  
  • Kobayashi, I., Muraoka, H., Iyoda, T., Nishida, M., Hasegawa, M., Yamaguchi, K. (2004). Antimicrobial susceptibility testing of vancomycin-resistant Enterococcus by the VITEK 2 system, and comparison with two NCCLS reference methods. J Med Microbiol 53: 1229-1232 [Abstract] [Full Text]  
  • Funke, G., Funke-Kissling, P. (2004). Evaluation of the New VITEK 2 Card for Identification of Clinically Relevant Gram-Negative Rods. J. Clin. Microbiol. 42: 4067-4071 [Abstract] [Full Text]  
  • de Cueto, M., Ceballos, E., Martinez-Martinez, L., Perea, E. J., Pascual, A. (2004). Use of Positive Blood Cultures for Direct Identification and Susceptibility Testing with the Vitek 2 System. J. Clin. Microbiol. 42: 3734-3738 [Abstract] [Full Text]  
  • Donay, J.-L., Mathieu, D., Fernandes, P., Pregermain, C., Bruel, P., Wargnier, A., Casin, I., Weill, F. X., Lagrange, P. H., Herrmann, J. L. (2004). Evaluation of the Automated Phoenix System for Potential Routine Use in the Clinical Microbiology Laboratory. J. Clin. Microbiol. 42: 1542-1546 [Abstract] [Full Text]  
  • Bruins, M. J., Bloembergen, P., Ruijs, G. J. H. M., Wolfhagen, M. J. H. M. (2004). Identification and Susceptibility Testing of Enterobacteriaceae and Pseudomonas aeruginosa by Direct Inoculation from Positive BACTEC Blood Culture Bottles into Vitek 2. J. Clin. Microbiol. 42: 7-11 [Abstract] [Full Text]  
  • O'Hara, C. M., Sowers, E. G., Bopp, C. A., Duda, S. B., Strockbine, N. A. (2003). Accuracy of Six Commercially Available Systems for Identification of Members of the Family Vibrionaceae. J. Clin. Microbiol. 41: 5654-5659 [Abstract] [Full Text]  
  • Ling, T. K. W., Liu, Z. K., Cheng, A. F. B. (2003). Evaluation of the VITEK 2 System for Rapid Direct Identification and Susceptibility Testing of Gram-Negative Bacilli from Positive Blood Cultures. J. Clin. Microbiol. 41: 4705-4707 [Abstract] [Full Text]  
  • Spanu, T., Sanguinetti, M., Ciccaglione, D., D'Inzeo, T., Romano, L., Leone, F., Fadda, G. (2003). Use of the VITEK 2 System for Rapid Identification of Clinical Isolates of Staphylococci from Bloodstream Infections. J. Clin. Microbiol. 41: 4259-4263 [Abstract] [Full Text]  
  • O'Hara, C. M., Miller, J. M. (2003). Evaluation of the Vitek 2 ID-GNB Assay for Identification of Members of the Family Enterobacteriaceae and Other Nonenteric Gram-Negative Bacilli and Comparison with the Vitek GNI+ Card. J. Clin. Microbiol. 41: 2096-2101 [Abstract] [Full Text]  
  • Barry, J., Brown, A., Ensor, V., Lakhani, U., Petts, D., Warren, C., Winstanley, T. (2003). Comparative evaluation of the VITEK 2 Advanced Expert System (AES) in five UK hospitals. J Antimicrob Chemother 51: 1191-1202 [Abstract] [Full Text]  
  • Horstkotte, M. A., Knobloch, J. K.-M., Rohde, H., Dobinsky, S., Mack, D. (2002). Rapid Detection of Methicillin Resistance in Coagulase-Negative Staphylococci with the VITEK 2 System. J. Clin. Microbiol. 40: 3291-3295 [Abstract] [Full Text]  
  • Ligozzi, M., Bernini, C., Bonora, M. G., de Fatima, M., Zuliani, J., Fontana, R. (2002). Evaluation of the VITEK 2 System for Identification and Antimicrobial Susceptibility Testing of Medically Relevant Gram-Positive Cocci. J. Clin. Microbiol. 40: 1681-1686 [Abstract] [Full Text]  
  • Brisse, S., Stefani, S., Verhoef, J., Van Belkum, A., Vandamme, P., Goessens, W. (2002). Comparative Evaluation of the BD Phoenix and VITEK 2 Automated Instruments for Identification of Isolates of the Burkholderia cepacia Complex. J. Clin. Microbiol. 40: 1743-1748 [Abstract] [Full Text]  
  • Livermore, D. M., Struelens, M., Amorim, J., Baquero, F., Bille, J., Canton, R., Henning, S., Gatermann, S., Marchese, A., Mittermayer, H., Nonhoff, C., Oakton, K. J., Praplan, F., Ramos, H., Schito, G. C., Van Eldere, J., Verhaegen, J., Verhoef, J., Visser, M. R. (2002). Multicentre evaluation of the VITEK 2 Advanced Expert System for interpretive reading of antimicrobial resistance tests. J Antimicrob Chemother 49: 289-300 [Abstract] [Full Text]  
  • Joyanes, P., del Carmen Conejo, M., Martinez-Martinez, L., Perea, E. J. (2001). Evaluation of the VITEK 2 System for the Identification and Susceptibility Testing of Three Species of Nonfermenting Gram-Negative Rods Frequently Isolated from Clinical Samples. J. Clin. Microbiol. 39: 3247-3253 [Abstract] [Full Text]  
  • Ling, T. K. W., Tam, P. C., Liu, Z. K., Cheng, A. F. B. (2001). Evaluation of VITEK 2 Rapid Identification and Susceptibility Testing System against Gram-Negative Clinical Isolates. J. Clin. Microbiol. 39: 2964-2966 [Abstract] [Full Text]  
  • Garcia-Garrote, F., Cercenado, E., Bouza, E. (2000). Evaluation of a New System, VITEK 2, for Identification and Antimicrobial Susceptibility Testing of Enterococci. J. Clin. Microbiol. 38: 2108-2111 [Abstract] [Full Text]  
  • Cantón, R., Pérez-Vázquez, M., Oliver, A., Sánchez Del Saz, B., Gutiérrez, M. O., Martínez-Ferrer, M., Baquero, F. (2000). Evaluation of the Wider System, a New Computer-Assisted Image-Processing Device for Bacterial Identification and Susceptibility Testing. J. Clin. Microbiol. 38: 1339-1346 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»