This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Touze, A.
Right arrow Articles by Coursaget, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Touze, A.
Right arrow Articles by Coursaget, P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 1998, p. 2046-2051, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

The L1 Major Capsid Protein of Human Papillomavirus Type 16 Variants Affects Yield of Virus-Like Particles Produced in an Insect Cell Expression System

Antoine Touze,1 Slimane El Mehdaoui,1,2 Pierre-Yves Sizaret,3 Christine Mougin,4 Nubia Muñoz,5 and Pierre Coursaget1,*

Institut de Virologie de Tours and CJF INSERM 93/09 Immunologie des Maladies Infectieuses, UFR des Sciences Pharmaceutiques "Philippe Maupas," 37200 Tours,1 Laboratoire de Microscopie Electronique, Faculté de Médecine de Tours, 37000 Tours,3 Laboratoire de Virologie, Faculté de Médecine et de Pharmacie, 25000 Besançon,4 and International Agency for Research on Cancer, 69372 Lyon Cedex 2,5 France, and Laboratoire de Biologie Moléculaire, Université des Sciences et de la Technologie "H. Boumedienne," BP 32, El-Alia, Algeria2

Received 16 October 1997/Returned for modification 9 March 1998/Accepted 2 April 1998

The L1 major capsid proteins of six human papillomavirus type 16 (HPV-16) strains were expressed in insect cells by using recombinant baculoviruses. Virus-like particles (VLPs) which appeared similar to empty virions were identified by electron microscopy for all HPV strains investigated. However, the yield of VLPs produced varied in a range from 1 to 79 depending on the HPV-16 strain. The L1 proteins of these strains differed by up to 15 amino acids from the L1 protein of the prototype HPV-16 strain. Mutations in the amino acid region from residues 83 to 97 seemed to affect the level of expression of the L1 protein. These results are important when considering the development of HPV vaccines and serological tests. They indicate that strains inducing high levels of VLP production must be selected for the development of vaccines. Moreover, the L1 proteins of all strains investigated were able to bind with DNA. We also investigated the seroreactivities of VLPs derived from three different HPV-16 strains from Algeria, Senegal, and the Philippines by testing sera from women from 11 countries in immunoglobulin G-specific enzyme-linked immunosorbent assays. We observed a strong correlation between the reactivities of the three different VLP variants, independent of the geographical origin of the sera investigated. These results indicate that the three strains investigated are serologically cross-reactive despite the fact that their L1 proteins differ in 14 amino acids and suggest that VLPs derived from only one HPV-16 strain could be sufficient for the development of an HPV-16 vaccine and anti-HPV-16 tests.

* Corresponding author. Mailing address: Faculté des Sciences Pharmaceutiques, 31 avenue Monge, 37200 Tours, France. Phone: 33.247.36.71.89. Fax: 33.247.36.71.88. E-mail: coursaget{at}univ-tours.fr.

Journal of Clinical Microbiology, July 1998, p. 2046-2051, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Chen, Z., DeSalle, R., Schiffman, M., Herrero, R., Burk, R. D. (2009). Evolutionary Dynamics of Variant Genomes of Human Papillomavirus Types 18, 45, and 97. J. Virol. 83: 1443-1455 [Abstract] [Full Text]  
  • Urquiza, M., Sanchez, R., Amaya, J., Leon, S., Acosta, J., Patarroyo, M. A., Camargo, M., Patarroyo, M. E. (2008). Specificity of L1 Peptides versus Virus-Like Particles for Detection of Human Papillomavirus-Positive Cervical Lesions in Females Attending Engativa Hospital, Bogota, Colombia. J. Clin. Microbiol. 46: 3714-3720 [Abstract] [Full Text]  
  • Pande, S., Jain, N., Prusty, B. K., Bhambhani, S., Gupta, S., Sharma, R., Batra, S., Das, B. C. (2008). Human Papillomavirus Type 16 Variant Analysis of E6, E7, and L1 Genes and Long Control Region in Biopsy Samples from Cervical Cancer Patients in North India. J. Clin. Microbiol. 46: 1060-1066 [Abstract] [Full Text]  
  • Fleury, M. J. J., Touze, A., de Sanjose, S., Bosch, F. X., Klaustermeiyer, J., Coursaget, P. (2008). Detection of Human Papillomavirus Type 31-Neutralizing Antibodies from Naturally Infected Patients by an Assay Based on Intracellular Assembly of Luciferase-Expressing Pseudovirions. CVI 15: 172-175 [Abstract] [Full Text]  
  • Ryding, J., Dahlberg, L., Wallen-Ohman, M., Dillner, J. (2007). Deletion of a major neutralizing epitope of human papillomavirus type 16 virus-like particles. J. Gen. Virol. 88: 792-802 [Abstract] [Full Text]  
  • Bousarghin, L., Hubert, P., Franzen, E., Jacobs, N., Boniver, J., Delvenne, P. (2005). Human papillomavirus 16 virus-like particles use heparan sulfates to bind dendritic cells and colocalize with langerin in Langerhans cells. J. Gen. Virol. 86: 1297-1305 [Abstract] [Full Text]  
  • Zhao, K.-N., Frazer, I. H. (2002). Saccharomyces cerevisiae Is Permissive for Replication of Bovine Papillomavirus Type 1. J. Virol. 76: 12265-12273 [Abstract] [Full Text]  
  • Combita, A.-L., Touze, A., Bousarghin, L., Christensen, N. D., Coursaget, P. (2002). Identification of Two Cross-Neutralizing Linear Epitopes within the L1 Major Capsid Protein of Human Papillomaviruses. J. Virol. 76: 6480-6486 [Abstract] [Full Text]  
  • Bousarghin, L., Combita-Rojas, A.-L., Touze, A., El Mehdaoui, S., Sizaret, P.-Y., Bravo, M.-M., Coursaget, P. (2002). Detection of Neutralizing Antibodies against Human Papillomaviruses (HPV) by Inhibition of Gene Transfer Mediated by HPV Pseudovirions. J. Clin. Microbiol. 40: 926-932 [Abstract] [Full Text]  
  • Bayo, S., Bosch, F X., de Sanjose, S., Munoz, N., Combita, A. L., Coursaget, P., Diaz, M., Dolo, A., van den Brule, A. J., Meijer, C. J. (2002). Risk factors of invasive cervical cancer in Mali. Int J Epidemiol 31: 202-209 [Abstract] [Full Text]  
  • Touze, A., de Sanjose, S., Coursaget, P., Almirall, M. R., Palacio, V., Meijer, C. J. L. M., Kornegay, J., Bosch, F. X. (2001). Prevalence of Anti-Human Papillomavirus Type 16, 18, 31, and 58 Virus-Like Particles in Women in the General Population and in Prostitutes. J. Clin. Microbiol. 39: 4344-4348 [Abstract] [Full Text]  
  • Touze, A., Bousarghin, L., Ster, C., Combita, A.-L., Roingeard, P., Coursaget, P. (2001). Gene transfer using human polyomavirus BK virus-like particles expressed in insect cells. J. Gen. Virol. 82: 3005-3009 [Abstract] [Full Text]  
  • El Mehdaoui, S., Touzé, A., Laurent, S., Sizaret, P.-Y., Rasschaert, D., Coursaget, P. (2000). Gene Transfer Using Recombinant Rabbit Hemorrhagic Disease Virus Capsids with Genetically Modified DNA Encapsidation Capacity by Addition of Packaging Sequences from the L1 or L2 Protein of Human Papillomavirus Type 16. J. Virol. 74: 10332-10340 [Abstract] [Full Text]  
  • Dupuy, C., Buzoni-Gatel, D., Touze, A., Bout, D., Coursaget, P. (1999). Nasal Immunization of Mice with Human Papillomavirus Type 16 (HPV-16) Virus-Like Particles or with the HPV-16 L1 Gene Elicits Specific Cytotoxic T Lymphocytes in Vaginal Draining Lymph Nodes. J. Virol. 73: 9063-9071 [Abstract] [Full Text]  
  • Caparros-Wanderley, W, Savage, N, Hill-Perkins, M, Layton, G, Weber, J, Davies, D. (1999). Intratype sequence variation among clinical isolates of the human papillomavirus type 6 L1 ORF: clustering of mutations and identification of a frequent amino acid sequence variant. J. Gen. Virol. 80: 1025-1033 [Abstract]  
  • Touze, A., Enogat, N., Buisson, Y., Coursaget, P. (1999). Baculovirus Expression of Chimeric Hepatitis B Virus Core Particles with Hepatitis E Virus Epitopes and Their Use in a Hepatitis E Immunoassay. J. Clin. Microbiol. 37: 438-441 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»