This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pinjon, E.
Right arrow Articles by Coleman, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pinjon, E.
Right arrow Articles by Coleman, D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 1998, p. 2093-2095, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Simple, Inexpensive, Reliable Method for Differentiation of Candida dubliniensis from Candida albicans

Emmanuelle Pinjon,1 Derek Sullivan,1 Ira Salkin,2,* Diarmuid Shanley,1 and David Coleman1

Department of Oral Medicine and Pathology, School of Dental Science and Dublin Dental Hospital, Trinity College, University of Dublin, Dublin 2, Republic of Ireland,1 and Wadsworth Center, New York State Department of Health, Albany, New York2

Received 13 February 1998/Accepted 21 April 1998

Candida dubliniensis is a recently described pathogenic species which shares many phenotypic features with Candida albicans, including the ability to form germ tubes and chlamydospores. These similarities have caused significant problems in the identification of C. dubliniensis by the average clinical mycology laboratory. To facilitate the differentiation of these species, we investigated the growth of 120 isolates of C. dubliniensis and 98 C. albicans isolates at 42 and 45°C on Emmons' modified Sabouraud glucose agar (SGA) and 10 isolates of each species in yeast-peptone-dextrose broth. None of the C. dubliniensis isolates grew on the agar or in the broth medium at 45°C, while 11 isolates were capable of growing on SGA at 42°C. In contrast, all of the C. albicans isolates but one grew at 45°C on or in either medium. These reproducible results clearly demonstrate that the incubation of isolates suspected to be C. dubliniensis or C. albicans at 45°C provides a simple, reliable, and inexpensive method for the differentiation of the two species.

* Corresponding author. Mailing address: Wadsworth Center, New York State Department of Health, Albany, NY 12201-0509. Phone: (518) 485-5386. Fax: (518) 485 5414. E-mail: ifs02{at}health.state.ny.us.

Journal of Clinical Microbiology, July 1998, p. 2093-2095, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • McManus, B. A., Coleman, D. C., Moran, G., Pinjon, E., Diogo, D., Bougnoux, M.-E., Borecka-Melkusova, S., Bujdakova, H., Murphy, P., d'Enfert, C., Sullivan, D. J. (2008). Multilocus Sequence Typing Reveals that the Population Structure of Candida dubliniensis Is Significantly Less Divergent than That of Candida albicans. J. Clin. Microbiol. 46: 652-664 [Abstract] [Full Text]  
  • Li, J., Xu, Y.-C., Bai, F.-Y. (2006). Candida pseudorugosa sp. nov., a Novel Yeast Species from Sputum. J. Clin. Microbiol. 44: 4486-4490 [Abstract] [Full Text]  
  • Patel, M., Shackleton, J. T., Coogan, M. M. (2006). Effect of antifungal treatment on the prevalence of yeasts in HIV-infected subjects.. J Med Microbiol 55: 1279-1284 [Abstract] [Full Text]  
  • Marot-Leblond, A., Beucher, B., David, S., Nail-Billaud, S., Robert, R. (2006). Development and Evaluation of a Rapid Latex Agglutination Test Using a Monoclonal Antibody To Identify Candida dubliniensis Colonies. J. Clin. Microbiol. 44: 138-142 [Abstract] [Full Text]  
  • Goldenberg, O., Herrmann, S., Adam, T., Marjoram, G., Hong, G., Gobel, U. B., Graf, B. (2005). Use of Denaturing High-Performance Liquid Chromatography for Rapid Detection and Identification of Seven Candida Species. J. Clin. Microbiol. 43: 5912-5915 [Abstract] [Full Text]  
  • Sahand, I. H., Moragues, M. D., Eraso, E., Villar-Vidal, M., Quindos, G., Ponton, J. (2005). Supplementation of CHROMagar Candida Medium with Pal's Medium for Rapid Identification of Candida dubliniensis. J. Clin. Microbiol. 43: 5768-5770 [Abstract] [Full Text]  
  • Al Mosaid, A., Sullivan, D. J., Polacheck, I., Shaheen, F. A., Soliman, O., Al Hedaithy, S., Al Thawad, S., Kabadaya, M., Coleman, D. C. (2005). Novel 5-Flucytosine-Resistant Clade of Candida dubliniensis from Saudi Arabia and Egypt Identified by Cd25 Fingerprinting. J. Clin. Microbiol. 43: 4026-4036 [Abstract] [Full Text]  
  • Davis, L. E., Shields, C. E., Merz, W. G. (2005). Use of a Commercial Reagent Leads to Reduced Germ Tube Production by Candida dubliniensis. J. Clin. Microbiol. 43: 2465-2466 [Abstract] [Full Text]  
  • Marot-Leblond, A., Grimaud, L., David, S., Sullivan, D. J., Coleman, D. C., Ponton, J., Robert, R. (2004). Evaluation of a Rapid Immunochromatographic Assay for Identification of Candida albicans and Candida dubliniensis. J. Clin. Microbiol. 42: 4956-4960 [Abstract] [Full Text]  
  • Moran, G., Stokes, C., Thewes, S., Hube, B., Coleman, D. C., Sullivan, D. (2004). Comparative genomics using Candida albicans DNA microarrays reveals absence and divergence of virulence-associated genes in Candida dubliniensis. Microbiology 150: 3363-3382 [Abstract] [Full Text]  
  • Al Mosaid, A., Sullivan, D. J., Coleman, D. C. (2003). Differentiation of Candida dubliniensis from Candida albicans on Pal's Agar. J. Clin. Microbiol. 41: 4787-4789 [Abstract] [Full Text]  
  • Fotedar, R., Al Hedaithy, S. S. A. (2003). Candida dubliniensis at a University Hospital in Saudi Arabia. J. Clin. Microbiol. 41: 1907-1911 [Abstract] [Full Text]  
  • Bliss, J. M., Sullivan, M. A., Malone, J., Haidaris, C. G. (2003). Differentiation of Candida albicans and Candida dubliniensis by Using Recombinant Human Antibody Single-Chain Variable Fragments Specific for Hyphae. J. Clin. Microbiol. 41: 1152-1160 [Abstract] [Full Text]  
  • Mosca, C. O., Moragues, M. D., Llovo, J., Al Mosaid, A., Coleman, D. C., Ponton, J. (2003). Casein Agar: a Useful Medium for Differentiating Candida dubliniensis from Candida albicans. J. Clin. Microbiol. 41: 1259-1262 [Abstract] [Full Text]  
  • Fitzgerald, D. H., Coleman, D. C., O'Connell, B. C. (2003). Susceptibility of Candida dubliniensis to Salivary Histatin 3. Antimicrob. Agents Chemother. 47: 70-76 [Abstract] [Full Text]  
  • Sancak, B., Rex, J. H., Paetznick, V., Chen, E., Rodriguez, J. (2003). Evaluation of a Method for Identification of Candida dubliniensis Bloodstream Isolates. J. Clin. Microbiol. 41: 489-491 [Abstract] [Full Text]  
  • Xu, J, Millar, B C, Moore, J E, McClurg, R, Walker, M J, Evans, J, Hedderwick, S, McMullan, R (2002). Comparison of API20C with molecular identification of Candida spp isolated from bloodstream infections. J. Clin. Pathol. 55: 774-777 [Abstract] [Full Text]  
  • Gee, S. F., Joly, S., Soll, D. R., Meis, J. F. G. M., Verweij, P. E., Polacheck, I., Sullivan, D. J., Coleman, D. C. (2002). Identification of Four Distinct Genotypes of Candida dubliniensis and Detection of Microevolution In Vitro and In Vivo. J. Clin. Microbiol. 40: 556-574 [Abstract] [Full Text]  
  • Oliveira, K., Haase, G., Kurtzman, C., Hyldig-Nielsen, J. J., Stender, H. (2001). Differentiation of Candida albicans and Candida dubliniensis by Fluorescent In Situ Hybridization with Peptide Nucleic Acid Probes. J. Clin. Microbiol. 39: 4138-4141 [Abstract] [Full Text]  
  • Ramage, G., Vande Walle, K., Wickes, B. L., Lopez-Ribot, J. L. (2001). Biofilm Formation by Candida dubliniensis. J. Clin. Microbiol. 39: 3234-3240 [Abstract] [Full Text]  
  • Staib, P., Moran, G. P., Sullivan, D. J., Coleman, D. C., Morschhäuser, J. (2001). Isogenic Strain Construction and Gene Targeting in Candida dubliniensis. J. Bacteriol. 183: 2859-2865 [Abstract] [Full Text]  
  • Al Mosaid, A., Sullivan, D., Salkin, I. F., Shanley, D., Coleman, D. C. (2001). Differentiation of Candida dubliniensis from Candida albicans on Staib Agar and Caffeic Acid-Ferric Citrate Agar. J. Clin. Microbiol. 39: 323-327 [Abstract] [Full Text]  
  • Stevens, D. A., Coleman, D. C., Sullivan, D. J. (2001). First Report of Candida dubliniensis in the Middle East. J. Clin. Microbiol. 39: 416-416 [Full Text]  
  • Ruhnke, M., Schmidt-Westhausen, A., Morschhauser, J. (2000). Development of simultaneous resistance to fluconazole in Candida albicans and Candida dubliniensis in a patient with AIDS. J Antimicrob Chemother 46: 291-295 [Abstract] [Full Text]  
  • Park, S., Wong, M., Marras, S. A. E., Cross, E. W., Kiehn, T. E., Chaturvedi, V., Tyagi, S., Perlin, D. S. (2000). Rapid Identification of Candida dubliniensis Using a Species-Specific Molecular Beacon. J. Clin. Microbiol. 38: 2829-2836 [Abstract] [Full Text]  
  • Meis, J. F. G. M., Verduyn Lunel, F. M., Verweij, P. E., Voss;, A., Coleman, D. (2000). One-Year Prevalence of Candida dublinienis in a Dutch University Hospital. J. Clin. Microbiol. 38: 3139-3140 [Full Text]  
  • Jabra-Rizk, M. A., Falkler, W. A. Jr., Merz, W. G., Baqui, A. A. M. A., Kelley, J. I., Meiller, T. F. (2000). Retrospective Identification and Characterization of Candida dubliniensis Isolates among Candida albicans Clinical Laboratory Isolates from Human Immunodeficiency Virus (HIV)-Infected and Non-HIV-Infected Individuals. J. Clin. Microbiol. 38: 2423-2426 [Abstract] [Full Text]  
  • Tintelnot, K., Haase, G., Seibold, M., Bergmann, F., Staemmler, M., Franz, T., Naumann, D. (2000). Evaluation of Phenotypic Markers for Selection and Identification of Candida dubliniensis. J. Clin. Microbiol. 38: 1599-1608 [Abstract] [Full Text]  
  • Marot-Leblond, A., Grimaud, L., Nail, S., Bouterige, S., Apaire-Marchais, V., Sullivan, D. J., Robert, R. (2000). New Monoclonal Antibody Specific for Candida albicans Germ Tube. J. Clin. Microbiol. 38: 61-67 [Abstract] [Full Text]  
  • Polacheck, I., Strahilevitz, J., Sullivan, D., Donnelly, S., Salkin, I. F., Coleman, D. C. (2000). Recovery of Candida dubliniensis from Non-Human Immunodeficiency Virus-Infected Patients in Israel. J. Clin. Microbiol. 38: 170-174 [Abstract] [Full Text]  
  • Gales, A. C., Pfaller, M. A., Houston, A. K., Joly, S., Sullivan, D. J., Coleman, D. C., Soll, D. R. (1999). Identification of Candida dubliniensis Based on Temperature and Utilization of Xylose and alpha -Methyl-D-Glucoside as Determined with the API 20C AUX and Vitek YBC Systems. J. Clin. Microbiol. 37: 3804-3808 [Abstract] [Full Text]  
  • Pincus, D. H., Coleman, D. C., Pruitt, W. R., Padhye, A. A., Salkin, I. F., Geimer, M., Bassel, A., Sullivan, D. J., Clarke, M., Hearn, V. (1999). Rapid Identification of Candida dubliniensis with Commercial Yeast Identification Systems. J. Clin. Microbiol. 37: 3533-3539 [Abstract] [Full Text]  
  • Kellogg, J. A., Bankert, D. A., Chaturvedi, V. (1999). Variation in Microbial Identification System Accuracy for Yeast Identification Depending on Commercial Source of Sabouraud Dextrose Agar. J. Clin. Microbiol. 37: 2080-2083 [Abstract] [Full Text]  
  • Jabra-Rizk, M. A., Falkler, W. A. Jr., Merz, W. G., Kelley, J. I., Baqui, A. A. M. A., Meiller, T. F. (1999). Coaggregation of Candida dubliniensis with Fusobacterium nucleatum. J. Clin. Microbiol. 37: 1464-1468 [Abstract] [Full Text]  
  • Bauters, T. G., Peleman, R., Moerman, M., Vermeersch, H., de Looze, D., Noens, L., Nelis, H. J. (1999). Membrane Filtration Test for Rapid Presumptive Differentiation of Four Candida Species. J. Clin. Microbiol. 37: 1498-1502 [Abstract] [Full Text]  
  • Kurzai, O., Heinz, W. J., Sullivan, D. J., Coleman, D. C., Frosch, M., Mühlschlegel, F. A. (1999). Rapid PCR Test for Discriminating between Candida albicans and Candida dubliniensis Isolates Using Primers Derived from the pH-Regulated PHR1 and PHR2 Genes of C. albicans. J. Clin. Microbiol. 37: 1587-1590 [Abstract] [Full Text]  
  • Pfaller, M. A., Messer, S. A., Gee, S., Joly, S., Pujol, C., Sullivan, D. J., Coleman, D. C., Soll, D. R. (1999). In Vitro Susceptibilities of Candida dubliniensis Isolates Tested against the New Triazole and Echinocandin Antifungal Agents. J. Clin. Microbiol. 37: 870-872 [Abstract] [Full Text]  
  • Jabra-Rizk, M. A., Baqui, A. A. M. A., Kelley, J. I., Falkler, W. A. Jr., Merz, W. G., Meiller, T. F. (1999). Identification of Candida dubliniensis in a Prospective Study of Patients in the United States. J. Clin. Microbiol. 37: 321-326 [Abstract] [Full Text]  
  • Koehler, A. P., Chu, K.-C., Houang, E. T. S., Cheng, A. F. B. (1999). Simple, Reliable, and Cost-Effective Yeast Identification Scheme for the Clinical Laboratory. J. Clin. Microbiol. 37: 422-426 [Abstract] [Full Text]  
  • Elie, C. M., Lott, T. J., Reiss, E., Morrison, C. J. (1998). Rapid Identification of Candida Species with Species-Specific DNA Probes. J. Clin. Microbiol. 36: 3260-3265 [Abstract] [Full Text]  
  • Odds, F. C., Van Nuffel, L., Dams, G. (1998). Prevalence of Candida dubliniensis Isolates in a Yeast Stock Collection. J. Clin. Microbiol. 36: 2869-2873 [Abstract] [Full Text]  
  • Bikandi, J., Millán, R. S., Moragues, M. D., Cebas, G., Clarke, M., Coleman, D. C., Sullivan, D. J., Quindós, G., Pontón, J. (1998). Rapid Identification of Candida dubliniensis by Indirect Immunofluorescence Based on Differential Localization of Antigens on C. dubliniensis Blastospores and Candida albicans Germ Tubes. J. Clin. Microbiol. 36: 2428-2433 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»