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Journal of Clinical Microbiology, July 1998, p. 2105-2108, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.

Genetic Analysis of Multiple Vancomycin-Resistant Enterococcus Isolates Obtained Serially from Two Long-Term-Care Patients

Dianna J. Schoonmaker,1,* Lawrence H. Bopp,2,dagger Aldona L. Baltch,3 Raymond P. Smith,3 Mary Ellen Rafferty,3 and Mary George4

Wadsworth Center, New York State Department of Health,1 and Infectious Disease Section3 and Laboratory Service,4 Stratton Veterans Affairs Medical Center and Albany Medical College, Albany, New York 12208, and Department of Biological Sciences, University of Southern Mississippi, Hattiesburg, Mississippi 394062

Received 2 December 1997/Returned for modification 14 January 1998/Accepted 24 April 1998

Fifty-eight vancomycin-resistant enterococcal isolates were obtained from two patients over 9 weeks. Numerous pulsed-field gel electrophoresis fingerprinting types were isolated from each patient. By PCR, all isolates were vanA+. However, many isolates from patient B were found to lack vanA by hybridization. Our results demonstrate the importance of examining multiple isolates, especially from patients who are at high risk of infection.

* Corresponding author. Mailing address: Wadsworth Center, New York State Department of Health, 120 New Scotland Ave., Albany, NY 12208. Phone: (518) 474-5822. Fax: (518) 473-6150. E-mail: djs03{at}health.state.ny.us.

dagger Present address: Biology Department, Siena College, Loudonville, NY 12211.

Journal of Clinical Microbiology, July 1998, p. 2105-2108, Vol. 36, No. 7
0095-1137/98/$04.00+0
Copyright © 1998, American Society for Microbiology. All rights reserved.


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  • Suppola, J. P., Kolho, E., Salmenlinna, S., Tarkka, E., Vuopio-Varkila, J., Vaara, M. (1999). vanA and vanB Incorporate into an Endemic Ampicillin-Resistant Vancomycin-Sensitive Enterococcus faecium Strain: Effect on Interpretation of Clonality. J. Clin. Microbiol. 37: 3934-3939 [Abstract] [Full Text]  
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